Naphthalenesulfonic acid, di-C9-rich C8-10-branched alkyl derivs., barium salts

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 25 - March 10, 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was not conducted to meet GLP regulations, however, the test followed standard guideline procedures (US Federal Hazardous Substances Labeling Act (FHSLA) 16 CFR 1500).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Acute Dermal Toxicity: FHSLA 16 CFR 1500.

Deviations:

not specified

GLP compliance:

no

Test type:

other: Limit test

Test material

Test material information

Constituent 1

Reference substance name:

barium bis(di C8-C10, branched, C9 rich, alkylnaphthalenesulphonate)

EC Number:

939-718-2

Cas Number:

1474044-80-8

Molecular formula:

C28H44O3S.1/2Ba

IUPAC Name:

barium bis(di C8-C10, branched, C9 rich, alkylnaphthalenesulphonate)

Test material form:

other: as solution in light paraffinic and naphthenic petroleum distillates

Details on test material:

A viscous brown liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

During the test period the animals were housed In Individual stainless steel wire bottomed cages in an environmentally controlled room. The
temperature was kept at 68 - 72 F. They were exposed to 12 hours of fluorescent light (8 A.M. - 8 P.M.) and 12 hours of darkness (8 P.M. -
8 A.M.) dai Iy. The animals were housed 1 per cage. Feed and water were provided ad-libitum after dosing.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The rabbits were prepared by clipping the skin of the trunk free of hair. Epidermal abrasions were made over a 5-6 cm2 area on 3 males and 2 females. The remaining five rabbits were not abraded. A non-permeable patch containing 2.0 ml/kg body weight of the test material was placed over a 4-5 cm2 area on all rabbits. The patches were secured in place with tape and an elastic sleeve. The rabbits were immobilized in head stocks for 24 hours at which time the patches were removed and the rabbits returned to their cages. Feed and water were provided ad-libitum. The rabbits were
observed for 14 days.

Duration of exposure:

24 hours

Results and discussion

Preliminary study:

A previouslyconducted study (Product Safety Labs Report No. T-348, April 4, 1978 ) demonstrated that application of a dermal dose of 20 ml/kg
the test substance produced 50% mortality.

Skin Sex Body Weight Dose Mortality
Treatment kg grams Test Day
Abraded M 2.7 54 2
Abraded M 2.8 56
Unabraded M 2.7 54 14
Unabraded M 2.7 54 3
Unabraded M 2.7 54 1
Abraded F 2.8 56 2
Abraded F 2.7 54 1
Abraded F 2.8 56 2
Unabraded F 2.8 56
Unabraded F 3.0 60 2

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 mL/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 000 mg/kg bw

Based on:

act. ingr.

Mortality:

No mortalities.

Clinical signs:

other: No signs of toxicity.

Gross pathology:

Not conducted due to lack of mortality.

Any other information on results incl. tables

Rabbit-No.	Sex	Body Weight-Initial kg	Body Weight-Final kg	Dose ml/kg
3527-abraded	M	2.83	2.83	5.7
3528-abraded	M	2.58	2.71	5.2
3529-abraded	M	2.54	2.78	5.1
3530	M	2.80	3.19	5.6
3531	M	3.34	3.35	6.7
3576	F	2.68	3.03	5.4
3577-abraded	F	2.44	2.58	4.9
3578-abraded	F	2.32	2.45	4.6
3478	F	3.32	3.48	6.6
3479	F	3.00	3.00	6.0

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

The Dermal LD50 of the test material is greater than 2.0 ml/kg for a 50% formulation. The LD50 as active ingredient is greater than 1000 mg/kg.

Executive summary:

A dermal acute toxicity test was conducted in New Zealand Albino Rabbits. Ten rabbits (5 male / 5 female) received a dose of 2 ml/kg bw of the test substance for 24 hours and were observed for 14 days for signs of toxicity and mortality. The skin of 5 rabbits was abraded prior to dosing (5-6 cm2 area on 3 males and 2 females). The Dermal LD50 of the test material is greater than 2.0 ml/kg. No mortality or toxic symptoms were observed. The LD50 as active ingredient is greater than 1000 mg/kg.