1,8-bis(phenylthio)anthraquinone

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

full method description is not available

GLP compliance:

no

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Div. Lab. Vers.
- Expiration date of the lot/batch: 11/2003

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature
- Solubility and stability of the test substance in the solvent/vehicle: <0.2 g/l (in water)

Analytical monitoring:

not specified

Details on sampling:

no data

Vehicle:

not specified

Details on test solutions:

no data

Test organisms (species):

other: bacteria

Details on inoculum:

no data

Test type:

not specified

Water media type:

not specified

Total exposure duration:

3 h

Post exposure observation period:

none

Hardness:

no data

Test temperature:

no data

pH:

8-8.1

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

Nominal: 64, 160, 400 and 1000 mg/L

Details on test conditions:

no data

Reference substance (positive control):

yes

Remarks:

3.5-Dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Due to its physico-chemical properties the test substance was not completely soluble in the test medium. The reported results are related to the amount of the test substance given into the test system.

Details on results:

none

Results with reference substance (positive control):

3h IC50 of reference substance (3,5 Dichlorophenol), was 13 mg/L.

Reported statistics and error estimates:

no data

Table:

Test substance (mg/L)	pH after 3 h	O2 consumption (in cm)	Time (in cm)	mg O2 pro min./L	% of control	% inhibition
1000	8.1	7.1	7.0	0.81	99.91	0.09
400	8.1	6.6	6.8	0.78	95.61	4.39
160	8.1	6.3	6.0	0.84	103.43	0.00
64	8.0	5.8	5.6	0.83	102. 03	0.00

Validity criteria fulfilled:

not specified

Conclusions:

The IC20 and IC50 (3 h) values of the test substance were determined to be >1000 mg/L (i.e. 960 mg a.i./L).

Executive summary:

The bacterial toxicity potential of FAT 93450/A was assessed in an activated sludge respiration inhibition test conducted according to OECD Guideline 209. The test substance was not completely soluble in the test medium. The reported results are based on nominal concentrations. The % inhibition observed was 0.09, 4.39, 0 and 0 % at 1000, 400, 160 and 64 mg/L concentration, respectively. Hence, the 3 h IC20 and IC50 values of the test substance were determined to be >1000 mg/L (i.e. 960 mg a.i./L). 

Description of key information

The bacterial toxicity potential of FAT 93450/A was assessed in an activated sludge respiration inhibition test conducted according to OECD Guideline 209. The test substance was not completely soluble in the test medium. The reported results are based on nominal concentrations. The % inhibition observed was 0.09, 4.39, 0 and 0 % at 1000, 400, 160 and 64 mg/L concentration, respectively. Hence, the 3 h IC20 and IC50 values of the test substance were determined to be >1000 mg/L (i.e. 960 mg a.i./L). 

Key value for chemical safety assessment

EC50 for microorganisms:

960 mg/L