1,8-bis(phenylthio)anthraquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Details on test conditions:

Nominal: 100, 1000 and 10000 mg/L

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

650 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No observed effect concentration: 100 mg/L
Maximum concentration tested causing no mortality: 100 mgIL
Minimum concentration tested causing 100 % mortality: 10000 mg/L

Sublethal observations / clinical signs:

The below symptoms were monitored after 1, 4, 24, 48, 72, 96 hours. Symptoms like apathy, swimming near water surface, narcotic like state, swimming near the bottom

NOMINAL CONC. (MG/L)	1H	4H	24H	48H	72H	96H
100
1000				CDA	CNA	CN
10000

A= apathy

C= swimming near water surface,

D= narcotic like state,

N- swimming near the bottom

Results:

 

Nominal conc	Number of fish	Dead fish after
(mg/L)		1 h	4h	24h	48h	72h	96h
100	10	0	0	0	0	0	0
1000	10	0	0	0	1	3	7
10000	10	0	10	10	10	10	10

Validity criteria fulfilled:

not specified

Conclusions:

The 96h LC50 for zebra fish (Danio rerio) was 650 mg/L (value of interpolation).

Executive summary:

The short term toxicity of the test substance was assessed in a study conducted according to a methodology equivalent to OECD Guideline 203. Groups of 10 Zebra fish each, were exposed to test substance at 100, 1000 or 10000 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h.

There was no mortality at 100 mg/L. 10 out of 10 fish died within 96 h at 10000 mg/L, while 7 out of 10 fish died after 96h at 1000 mg/L.

Based on the findings of the study, the 96 h LC50 for zebra fish (Danio rerio) was 650 mg/L (value of interpolation) and NOEC value (96h) was 100 mg/L.

Description of key information

The short term toxicity of the test substance was assessed in a study conducted according to a methodology equivalent to OECD Guideline 203. Groups of 10 Zebra fish each, were exposed to test substance at 100, 1000 or 10000 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h. There was no mortality at 100 mg/L. 10 out of 10 fish died within 96h at 10000 mg/L, while 7 out of 10 fish died after 96h at 1000 mg/L. Based on the findings of the study, the 96h LC50 for zebra fish (Danio rerio) was 650 mg/L (value of interpolation) and NOEC value (96h) was 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

650 mg/L