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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation
Remarks:
other: human data
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Peer reviewed literature data

Data source

Reference
Reference Type:
publication
Title:
The Mammalian Toxicity of Methacrylonitrile
Author:
Pozzani UC, Kinkead ER & King JM
Year:
1968
Bibliographic source:
American Industrial Hygiene Association Journal 29:202-210

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: no data
Deviations:
not specified
Principles of method if other than guideline:
Groups of eight to nine human subjects were exposed by inhalation twice to a series of methacrylonitrile concentrations for periods of 1 minute each, with 45-minute or longer intervals between exposures. The exposure concentrations were 24, 14, 0, 7, 14, 24, 7, 2, 0 and 2 ppm (in that order).
GLP compliance:
no
Remarks:
study predates GLP

Test material

Constituent 1
Chemical structure
Reference substance name:
Methacrylonitrile
EC Number:
204-817-5
EC Name:
Methacrylonitrile
Cas Number:
126-98-7
Molecular formula:
C4H5N
IUPAC Name:
methacrylonitrile
Details on test material:
- Name of test material (as cited in study report): Methacrylonitrile
- Source: Vistron Corporation, Midland Building, Cleveland, Ohio
- Molecular weight: 67.09
- Substance type: organic, monoconstituent
- Physical state: clear, colourless liquid
- Analytical purity: minimum of 99.0 wt %
- Boiling point: 90.3 degrees Centigrade at 760 mm
- Specific gravity: d 30°/4°, 0.7896
- Solubility in water: 2.54 wt % at 30 degrees Centigrade
- Flash point (Tag open cup): 55 degrees Fahrenheit
- Vapor pressure: 65 mm at 25 degrees Centigrade
- Inhibitor (MEHQ): 35-45 ppm
- Other: Air saturated with methacrylonitrile at 25 degrees Centigrade at 760 mm contains 85,526 ppm vapour, which has a relative vapour density of 1.11 (dry air=1)

Test animals / tissue source

Species:
human
Strain:
other: not applicable
Details on test animals or tissues and environmental conditions:
No data

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
In the first study, groups of eight to nine subjects were exposed to methacrylonitrile vapour at 0, 2, 7, 14 and 24 ppm. In the second study, subjects were exposed to 2 ppm or 14 ppm.
Duration of treatment / exposure:
First study - 1 min with intervals of 45 min or longer
Second study - 10 min
Observation period (in vivo):
No data
Number of animals or in vitro replicates:
First study - 7 to 9 subjects per exposure group
Second study - 9 subjects (2 ppm) or 7 subjects (14 ppm)

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Basis:
other: no data
Time point:
other: ≥ 1 min
Score:
0
Reversibility:
not specified
Remarks on result:
other: 22 % of subjects experienced eye irritation when exposed to 24 ppm for 1 min. No irritation was reported at lower concentrations.
Irritation parameter:
overall irritation score
Basis:
other: no data
Time point:
other: ≥ 10 min
Score:
0
Reversibility:
not specified
Remarks on result:
other: only 1-2 subjects experienced eye irritation when exposed to 2 ppm or 14 ppm for 10 min
Irritant / corrosive response data:
No data
Other effects:
First study - 6 % of subjects experienced nasal irritation when exposed to 24 ppm for 1 min
First study - 17 % of subjects experienced throat irritation when exposed to 24 ppm for 1 min
Second study - only 1-2 subjects experienced any form of nasal, throat or eye irritation

Applicant's summary and conclusion

Conclusions:
Eye irritation was experienced by 22 % of human subjects when exposed to 24 ppm for 1 min. No eye irritation was reported for 1 min exposure at at lower concentrations. However, methacrylonitrile caused irritation of the nose, throat or eyes in some subjects at concentrations of 2 ppm and 14 ppm when the exposure period was increased to 10 minutes.