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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1985-07-29- 1986-02-12
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report date:
1986

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
observation period was 7 days.
GLP compliance:
yes
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methacrylonitrile
EC Number:
204-817-5
EC Name:
Methacrylonitrile
Cas Number:
126-98-7
Molecular formula:
C4H5N
IUPAC Name:
methacrylonitrile
Constituent 2
Reference substance name:
2-methyl-2-propenenitrile
IUPAC Name:
2-methyl-2-propenenitrile
Details on test material:
- Physical state: Colorless liquid with cyanogen-like odour
- Lot/batch No.: 507-03-004B
- Source: supplied by Asahi Chemical Industry Co Ltd
- Storage condition of test material: in closed vessel at cold dark place

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Japan SLC, Inc.
- Age at study initiation: 5 weeks
- Weight at study initiation: 87-108 g

ENVIRONMENTAL CONDITIONS
- Temperature: 22 ± 2 degrees Centigrade
- Humidity: 55 ± 15 %
- Air changes (per hr): ventilated (all fresh air system) 15 times per hour
- Photoperiod (hrs dark / hrs light): 12-hour light-dark cycle (light on 8:00-20:00)

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: vehicle: 1%(v/v) Tween 80 in distilled water
Details on dermal exposure:
TEST SITE
- Area of exposure: back
- % coverage: 4 × 5 cm
- Type of wrap if used: Poly vinylidene chloride(PVDC) film for food use

REMOVAL OF TEST SUBSTANCE
- Washing: lukewarm water
- Time after start of exposure: 24 hr

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 10 mL/kg bodyweight
- Concentration (if solution): 0.5, 0.6, 0.8, 1.0, 1.2, 1.5, 1.9 mL/kg bodyweight
- Constant volume or concentration used: yes

VEHICLE
- Lot/batch no. (if required): ALE1780, Wako Pure Chemical Industries, Ltd. (Japan)
Duration of exposure:
24 hrs
Doses:
0.5, 0.6, 0.8, 1.0, 1.2, 1.5, 1.9 mL/kg bodyweight
No. of animals per sex per dose:
Seven female animals per dose level
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Statistics:
Probit method

Results and discussion

Preliminary study:
No data
Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
1.32 mL/kg bw
Based on:
test mat.
95% CL:
1.02 - 1.7
Mortality:
0.5 mL/kg: 0.0%
0.6 mL/kg: 0.0%
0.8 mL/kg: 14.3%
1.0 mL/kg: 28.6%
1.2 mL/kg: 57.1%
1.5 mL/kg: 42.9%
1.9 mL/kg: 100.0 %
Clinical signs:
Main signs:
Day 0: decrease in locomotor activity, ptosis, dyspnea, hypothermia, sadation, straub tail
Day 1: none of the signs described above
Body weight:
Day 1: decrease due to the treatment
Day 2 and thereafter: recovery
Gross pathology:
Dead animals: lung hemorrhage, subdural hemorrhage, slight erythema at the patch site
Survivors: no abnormalities
Other findings:
Cause of death was considered to be poisoning with hydrocyanic acid produced from the test substance in the body

Applicant's summary and conclusion

Conclusions:
LD50 was reported as 1.32 mL/kg bw in female rats