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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
cytotoxicity
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
no data
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Abstract only

Data source

Reference
Reference Type:
publication
Title:
Effect of methacrylonitrile on rat lung mitochondria and microsomes
Author:
Samikkannu T & Niranjali DS
Year:
1997
Bibliographic source:
Medical Science Research, 25(2): 129-132

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: no data
Deviations:
not specified
Principles of method if other than guideline:
Effects of methacrylonitrile on lung mitochondria and microsomes was studied using albino rats
GLP compliance:
not specified
Type of method:
in vivo
Endpoint addressed:
basic toxicokinetics

Test material

Constituent 1
Chemical structure
Reference substance name:
Methacrylonitrile
EC Number:
204-817-5
EC Name:
Methacrylonitrile
Cas Number:
126-98-7
Molecular formula:
C4H5N
IUPAC Name:
2-methylprop-2-enenitrile
Details on test material:
No data

Test animals

Species:
rat
Strain:
other: albino
Sex:
not specified
Details on test animals or test system and environmental conditions:
No data

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
other: sunflower oil
Details on exposure:
No data
Analytical verification of doses or concentrations:
not specified
Details on analytical verification of doses or concentrations:
No data
Duration of treatment / exposure:
Fourteen days
Frequency of treatment:
Daily
Post exposure period:
No data
Doses / concentrations
Remarks:
Doses / Concentrations:
100 mg/kg
Basis:
no data
No. of animals per sex per dose:
No data
Control animals:
not specified
Details on study design:
No data

Examinations

Examinations:
No data
Positive control:
No data

Results and discussion

Details on results:
Methacrylonitrile caused significant decrease in the activities of mitochondrial citric acid cycle enzymes and inner mitochondrial enzymes. The concentrations of cytochromes and the energy linked properties of lung mitochondria also declined. The respiratory control ratio was lowered in methacrylonitrile treated rats lung mitochondria, leading to a decrease in net energy product. In the microsomes, administration of methacrylonitrile resulted in significant increases in cytochrome-P450 and cytochrome b5 contents. The lung mitochondrial phospholipids and cholesterol and microsomal phospholipids were significantly lower.

Applicant's summary and conclusion

Conclusions:
Methacrylonitrile caused significant decrease in the activities of mitochondrial citric acid cycle enzymes and inner mitochondrial enzymes. The concentrations of cytochromes and the energy linked properties of lung mitochondria also declined. The respiratory control ratio was lowered in methacrylonitrile treated rats lung mitochondria, leading to a decrease in net energy product. In the microsomes, administration of methacrylonitrile resulted in significant increases in cytochrome-P450 and cytochrome b5 contents. The lung mitochondrial phospholipids and cholesterol and microsomal phospholipids were significantly lower.