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Diss Factsheets

Administrative data

Endpoint:
neurotoxicity: chronic oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Abstract only

Data source

Reference
Reference Type:
publication
Title:
Relative neurotoxicological properties of five unsaturated aliphatic nitriles in rats
Author:
Gagnaire F, Marignac B & Bonnet P
Year:
1988
Bibliographic source:
Journal of Applied Toxicology, 18(1): 25-31

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: no data
Deviations:
not specified
Principles of method if other than guideline:
Neurophysiological measurement during repeated dose oral administration of methacrylonitrile
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methacrylonitrile
EC Number:
204-817-5
EC Name:
Methacrylonitrile
Cas Number:
126-98-7
Molecular formula:
C4H5N
IUPAC Name:
methacrylonitrile
Details on test material:
No data

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
No data

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
not specified
Details on exposure:
Five days per week
Analytical verification of doses or concentrations:
not specified
Details on analytical verification of doses or concentrations:
No data
Duration of treatment / exposure:
Twelve weeks
Frequency of treatment:
Daily
Doses / concentrations
Remarks:
Doses / Concentrations:
50, 70 or 90 mg/kg bw
Basis:
no data
No. of animals per sex per dose:
No data
Control animals:
not specified
Details on study design:
Neurophysiological measurement was performed with a Racia-Medelec modular electrophysiological system, equipped with a DAV 62 computer.

Examinations

Observations and clinical examinations performed and frequency:
At least 16 hours after treatment during weeks 3, 6, 9, and 12 of exposure plus week 20 (8 weeks after exposure ended).
Specific biochemical examinations:
No data
Neurobehavioural examinations performed and frequency:
The motor conduction velocity of the tail nerves were measured together with the amplitudes of the sensory action potential and of the muscular action potential.
Sacrifice and (histo)pathology:
No data
Other examinations:
No data
Positive control:
No data
Statistics:
No data

Results and discussion

Results of examinations

Clinical signs:
effects observed, treatment-related
Description (incidence and severity):
mortality observed in 50 and 90 mg/kg groups
Mortality:
mortality observed, treatment-related
Description (incidence):
mortality observed in 50 and 90 mg/kg groups
Body weight and weight changes:
effects observed, treatment-related
Description (incidence and severity):
significant decrease in 70 and 90 mg/kg groups
Food consumption and compound intake (if feeding study):
not specified
Food efficiency:
not specified
Water consumption and compound intake (if drinking water study):
not specified
Ophthalmological findings:
not specified
Clinical biochemistry findings:
not specified
Behaviour (functional findings):
not specified
Gross pathological findings:
not specified
Neuropathological findings:
not specified
Other effects:
not specified
Description (incidence and severity):
Migrated information from 'Further observations for developmental neurotoxicity study'



Details on results (for developmental neurotoxicity):No data (migrated information)
Details on results:
Two rats died in the low dose group and eight rats died in the high dose group. Body weight was significantly decreased at 70 and 90 mg/kg. However, no abnormal behaviours were seen, and there were no significant changes in motor and senory conduction velocities and amplitudes of the sensory and motor potentials of the tail nerve.

Applicant's summary and conclusion

Conclusions:
There were no significant changes in motor and sensory conduction velocities and amplitudes of the sensory and motor potentials of the tail nerve during or after administration of methacrylonitrile to rats for 12 weeks.