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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP compliant non-guideline study, adequate for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The procedure followed in the present protocol mimic as closely as possible those described by Ames et al. (1975).
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
3,7-dimethyloct-6-enenitrile
EC Number:
257-288-8
EC Name:
3,7-dimethyloct-6-enenitrile
Cas Number:
51566-62-2
Molecular formula:
C10H17N
IUPAC Name:
3,7-dimethyloct-6-enenitrile
Details on test material:
- Name of test material: Citronellylnitril T-04526, No 167849, 16.9.80
No further data

Method

Target gene:
histidine gene
Species / strain
Species / strain / cell type:
S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA98, and TA 100
Metabolic activation:
with and without
Metabolic activation system:
S9 mix of Aroclor-induced rats
Test concentrations with justification for top dose:
0, 0.0007, 0.002, 0.007, 0.02, 0.06 mg test liquid per 0.1 mL methanol per plate.
Vehicle / solvent:
methanol
Controlsopen allclose all
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
sodium azide
Remarks:
Strains: TA 1535, TA 100, without metabolic activation
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: hycanthone methanesulphonate
Remarks:
Strains: TA 1537, TA 1538, TA 98, without metabolic activation
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: 2-aminoanthracene
Remarks:
all strains, with metabolic activation
Details on test system and experimental conditions:
METHOD OF APPLICATION: in agar (plate incorporation)

DURATION: After the top agar has been allowed to harden, the plates are incubated at 37°C for three days.

NUMBER OF REPLICATIONS: 3

DETERMINATION OF CYTOTOXICITY
- Method: inhibition of growth of the backgroun lawn
Evaluation criteria:
Not specifed
Statistics:
No data

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98, TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
Strain TA 1537
Vehicle controls validity:
valid
Untreated negative controls validity:
not examined
Positive controls validity:
valid
Additional information on results:
- The dose range used in the assay was based on a preliminary test performed to assess the toxicity of the compound for the bacteria. 0.1 mg test liquid per plate appeared slightly toxic as revealed by a less dense background lawn of bacterial growth.
- There were no signs that chemical toxicity interfered with the mutagenicity testing: minimal signs of chemical toxicity were only seen with strain TA 1537 in the presence of the S-9 mix at the highest level.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion