Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 257-288-8 | CAS number: 51566-62-2
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, comparable to current guideline, fully adequate for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- , occlusive testing
- GLP compliance:
- no
Test material
- Reference substance name:
- 3,7-dimethyloct-6-enenitrile
- EC Number:
- 257-288-8
- EC Name:
- 3,7-dimethyloct-6-enenitrile
- Cas Number:
- 51566-62-2
- Molecular formula:
- C10H17N
- IUPAC Name:
- 3,7-dimethyloct-6-enenitrile
- Details on test material:
- - Name of test material: Citronellylnitril.
- Test substance No.: 82/88.
- Stable until: March 1984.
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Gaukler, D-6050 Offenbach/Main, FRG.
- Weight at study initiation: males: 2.99 and 3.47 kg, female: 3.05 kg.
- Housing: Individually in stainless steel cages with wire mesh walk floor (floor area: 40 cm x 51 cm). No bedding in the cages; sawdust in the waste trays.
- Diet: Ovator Solikanin 4 mm; Muskator-Werke, D-4000 Düsseldorf 1, FRG (about 130 g per animal daily).
- Water: About 250 mL tap water per animal daily.
- Acclimation period: About 8 days before the beginning of the study in the skin laboratory; same housing conditions as during the study.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24.
- Humidity (%): 30 - 70.
- Photoperiod (hrs dark / hrs light): 12 h/12 h (6.00a.m. - 18.00p.m./18 p.m. - 8a.m.).
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- other: clipped
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated skin sites of the same animal
- Amount / concentration applied:
- 0.5 mL of the undiluted test liquid
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 8 days
- Number of animals:
- 3
- Details on study design:
- TEST SITE
- Clipping of fur at least 15 hours before the beginning of the study.
- Application area: 2.5 cm x 2.5 cm.
- Application site: Upper third of the region of the back or flank.
REMOVAL OF TEST SUBSTANCE
- Removal of the test substance: At the end of the exposure period with Lutrol and Lutrol/water (1 : 1).
OTHER
- Additional examinations: After sacrifice the animals are examined by gross pathology. If there are clinical signs of necroses, these are confirmed by
gross- pathological examination after incision of the skin. (If there are differences between the clinical and pathological assessments, only the latter is included in the final report.)
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- other: Mean of 24, 48 and 72 hours
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- other: Mean of 24, 48 and 72 hours
- Score:
- 0.67
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- other: Mean of 24, 48 and 72 hours
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- edema score
- Basis:
- animal: 1,2 and 3
- Time point:
- other: Mean of 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritant / corrosive response data:
- - After 72 hours one animal showed scaling in the abdominal and lateral position and dyspnea. This animal was death as a result of dysentery (no substance-related finding) 7 days after the study began.
- The two remaining animals showed scaling at day 8.
Any other information on results incl. tables
Score per animal
Readings |
Animal |
Erythema |
Edema |
4 hours |
1 |
1 |
0 |
|
2 |
1 |
0 |
|
3 |
1 |
0 |
24 hours |
1 |
1 |
0 |
|
2 |
1 |
0 |
|
3 |
1 |
0 |
48 hours |
1 |
0 |
0 |
|
2 |
0 |
0 |
|
3 |
0 |
0 |
72 hours |
1 |
0 |
0 |
|
2 |
1 |
0 |
|
3 |
0 |
0 |
8 days |
1 |
* |
* |
|
2 |
0 |
0 |
|
3 |
0 |
0 |
* animal died after 7days
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
