Registration Dossier

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP compliant non-guideline study, available as unpublished report, no restrictions, fully adequate for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 403.
A test group consisting of 10 animals/sex was treated by single inhalation application; aerosol of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy.
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3,7-dimethyloct-6-enenitrile
EC Number:
257-288-8
EC Name:
3,7-dimethyloct-6-enenitrile
Cas Number:
51566-62-2
Molecular formula:
C10H17N
IUPAC Name:
3,7-dimethyloct-6-enenitrile
Details on test material:
- Name of test material: Citronellylnitril
- Stability: At least 2 years under exclusion of oxygen

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Dr. K. Thomae GmbH, D-7950 Biberach, FRG
- Age at study initiation: 8 weeks
- Weight at study initiation: male animals 256 ± 22 g, female animals 176 ± 13 g.
- Housing: They were housed in groups of five in wire cages of Becker, type D III, without bedding
- Diet: The animais were offered maintenance diet for rats, mice and hamsters, SSNIFF R 10 mm pellet, SSNIFF-Versuchstierdiaten GmbH, D-4770 Soest, FRG.
- Water: Tap water ad libitum during the post-exposure observation period.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:
inhalation: aerosol
Type of inhalation exposure:
nose/head only
Vehicle:
air
Details on inhalation exposure:
TECHNICAL PROCEDURE:
- Exposure system: Head-nose inhalation system INA 20 (glass-steel construction, BASF Aktiengesellschaft, V ≈ 55 L); the animals are restrained in tubes and their snouts project into the inhalation chamber.
- Generator system: By means of a continuous infusion pump, INFU 362 (INDIGEL/Switzerland) and a two-component atomizer, mod. 970 (Schlick), a mixture of aerosol and air was generated.
- Experimental procedure: Constant amounts of the substance to be tested were supplied to a two-component atomizer by means of a metering pump. By means of compressed air (1.45 bar) an aerosol was generated, which was passed into the inhalation system. The exhaust air system was adjusted by 10% lower against the supply air system (positive pressure). This ensured that the mixture of test substance and air was not diluted by laboratory air in the breathing zones of the animals.

ANALYTICAL INVESTIGATIONS:
- Sampling: Apparatus: Quartz wool and a downstream fritted glass flask, BASF sampling station (with gas meter, impulse counter and automatic pump switching); Sorption solvent: 2-propanol; Sampling velocity: 1.25 m/s; Sampling amount: 1 L; Sampling site: immediately adjacent to the animal noses; Sampling probe ø: 7 mm; Sampling frequency: 1 sample every hour.
- Analytical method of determination: A gas-chromatographic method was used for the quantitative assay of the aerosol concentration. The aerosol samples were taken up in 40 mL 2-propanol (solvent). Gas-chromatographic method: GC HP/5840 A (Hewlett Packard). An internal standard was pipetted to the absorption sample contained in a 50 mL measuring flask and made up to the calibration mark. The chromatograpbic peak area was compared with calibration values and calculated.
- Particle size analysis: Equipment: Andersen Stack Sampler Mark III, Millipore vacuum compressed air pump XX 60 220 50, limiting orifice 3 L/min Millipore, sampling probe, internal diameter 6.9 mm, stopwatch. Procedure: 30 minutes after the beginning of the test at the earliest, one sample of the test group was taken for the particle size analysis. Before the sampling, the impactor was equipped with metal collecting discs and a backup particle filter. The impactor was connected to the pump and the test apparatus, and a sample (12 L) was removed. The impactor was taken apart, the collecting discs and the pre-impactor were rinsed with the solvent, the backup particle filter was eluted, and the samples obtained were analyzed by gas chromatography.
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
4.9 mg/L
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
Observation period: After the exposure period the surviving animals were observed for 14 days.
Clinical examinations: The body weight of the animals was checked before the beginning of the test, after 7 days and at the end of the observation period and was presented graphically. Clinical symptoms were recorded each workday. Mortality was checked each day.
Pathology: At the end of the 14-day observation period, the animals were sacrificed by CO2 and were subjected to a grosspathological examination.
Statistics:
The statistical evaluation of the concentration-response relationship was carried out in accordance with the binomial test (Wittig, H.: Mathematische Statistik 1974, pp. 32 - 35) according to tables of the BASF Computer Center.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 4.9 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Mortality:
No mortality was observed.
Clinical signs:
other: During exposure: aqueous discharge from noses, salivation. After exposure: aqueous, slight discharge from noses, salivation, reddened ears and limbs. After one day the animals were without findings.
Body weight:
The body weights of the test group were unimpaired on comparison to a control collective.
Gross pathology:
Organs: no abnormalities detected.
Other findings:
The MMAD 50% = 3.0 µm (geometrical standard deviation 2.9) was calculated from the results of the particle size analysis.
A fraction of 89% of the aerosol passing through the alveoli was obtained from the results of the particle size analysis.

Applicant's summary and conclusion