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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The study was conducted as per the method outlined in the publication - Alsop, G.M.; Waggy, G.T.; Conway, R.A., Bacterial Growth Inhibition Test, Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980 and the report contains sufficient information to permit a meaningful evaluation of study results
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study
Qualifier:
according to
Guideline:
other: Alsop, G.M.; Waggy, G.T.; Conway, R.A., Bacterial Growth Inhibition Test, Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980
Deviations:
no
Principles of method if other than guideline:
not applicable
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable
Analytical monitoring:
no
Details on sampling:
not applicable
Vehicle:
not specified
Details on test solutions:
no data
Test organisms (species):
other: no data
Details on inoculum:
no data
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Post exposure observation period:
not applicable
Hardness:
no data
Test temperature:
22 ± 2 °C
pH:
no data
Dissolved oxygen:
no data
Salinity:
not applicable
Nominal and measured concentrations:
no data
Details on test conditions:
The study was conducted as per the method outlined in the publication - Alsop, G.M.; Waggy, G.T.; Conway, R.A., Bacterial Growth Inhibition Test, Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980. The test material n-pentyl propionate was evaluated at selected conentrations in a mixture containing buffer, nutrients, growth substrate and microorganisms. The mixture of 1 ml of a suspension of seed microorganisms, 20 ml of dilution water, 4 ml of stock buffer solution, 10 ml of yeast extract/sodium acetate solution and 4 ml of aqueous solution of n-pentyl propionate were incubated in an 8-ounce, narrow neck, round bottle for 16 hours on a platform shaker at ambient temperature (22 ± 2 °C). Seeded control bottles were used to measure growth or turbidity generated during the 16 hours without the test material. The bottles were stoppered with cotton plugs during shaking to avoid contamination.
The degree of inhibition was assessed from measuring (optical density at 530 nm) the turbidity levels of the test material at various concentrations. The measured optical density values were calculated as a percentage of the seeded growth control system. The percentage of control values were then plotted against the log of test sample concentration. The test concentration corresponding to 50 percent of the control was termed as 50 percent inhibition concentration (IC50).
Reference substance (positive control):
no
Duration:
16 h
Dose descriptor:
IC50
Effect conc.:
240 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: turbidity measurements
Details on results:
no data
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
no data

None

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the study, the IC50 of n-pentyl propionate was 240 mg/l.
Executive summary:

The study was conducted as per the method outlined in the publication - Alsop, G.M.; Waggy, G.T.; Conway, R.A., Bacterial Growth Inhibition Test, Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980. The test material n-pentyl propionate was evaluated at selected conentrations in a mixture containing buffer, nutrients, growth substrate and microorganisms. The mixture of 1 ml of a suspension of seed microorganisms, 20 ml of dilution water, 4 ml of stock buffer solution, 10 ml of yeast extract/sodium acetate solution and 4 ml of aqueous solution of n-pentyl propionate were incubated in an 8-ounce, narrow neck, round bottle for 16 hours on a platform shaker at ambient temperature (22 ± 2 °C). Seeded control bottles were used to measure growth or turbidity generated during the 16 hours without the test material. The bottles were stoppered with cotton plugs during shaking to avoid contamination.

The degree of inhibition was assessed from measuring (optical density at 530 nm) the turbidity levels of the test material at various concentrations. The measured optical density values were calculated as a percentage of the seeded growth control system. The percentage of control values were then plotted against the log of test sample concentration. The test concentration corresponding to 50 percent of the control was termed as 50 percent inhibition concentration (IC50). Under the conditions of the study, the IC50 of n-pentyl propionate was 240 mg/l.

Description of key information

In a GLP study conducted as per methods laid out in the publication, "Bacterial Growth Inhibition Test", Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980, the 16-hour IC50 of pentyl propionate was 240 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
240 mg/L

Additional information

In a GLP study conducted as per methods laid out in the publication, "Bacterial Growth Inhibition Test", Journal Water Pollution Control Federation, Volume 52, No. 10, October 1980, the 16-hour IC50 of pentyl propionate was 240 mg/l.