Pentyl propionate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the method recommended by the EPA Committee on Methods for Toxicity Tests with aquatic organisms and the report contains sufficient information to permit a meaningful evaluation of study results

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: EPA Committee on Methods for Toxicity Tests with aquatic organisms

Deviations:

no

Principles of method if other than guideline:

not applicable

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Analytical monitoring:

no

Details on sampling:

not applicable

Vehicle:

not specified

Details on test solutions:

no data

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna
- Source: in-house culture, initially stocked from the EPA laboratory at Duluth, MN
- Age at study initiation: < 24 hours old
- Method of breeding: The culture was maintained at 19-23 °C in glass beakers filled with Kanawha river water. Culture transfers and feeding were performed three times a week and consisted of placing 20-30 young Daphnia into each of three 600 ml beakers of river water and fed with laboratory prepared feed cosisting of pulverized trout food, yeast and alfaalfa powder.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Hardness:

40-60 mg/l as CaCO3

Test temperature:

no data

pH:

7.0-7.2

Dissolved oxygen:

no data

Salinity:

not applicable

Nominal and measured concentrations:

no data

Details on test conditions:

The test procedures closely followed those recommended by the EPA Committee on Methods for Toxicity Tests with aquatic organisms, except that replicate concentrations were not always used. The doses for the definitive test was selected from 5-10 geometrically equidistant concentrations and included a control. The test was conducted in 250 ml beakers containing 200 ml of the test solution and 10 daphnia. The daphnid neonates (first instar) used in the test were less than 24 hours old which were obtained by isolating gravid females for approximately 20 hours.
kanawha river water was used in the preparation of test solutions and the physico-chemical characteristics revealed the water quality to be soft and was also used for maintaing the in-houe daphnid cultures.
Dissolved oxygen and pH were determined initially and at 48 hours for all test concentrations and controls. Mortalities were recorded at 24 and 48 hours.

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

60 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 50.6 - 71.2 mg/l

Details on results:

no data

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

The Trimmed Spearman-Karber program was used to calculate the 48-hour LC50 and 95 percent confidence intervals

None

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of the study, the 48-hour LC50 of n-pentyl propionate to daphnia magna was 60.0 mg/l with 95 percent confidence limits ranging between 50.6 - 71.2 mg/l.

Executive summary:

In this study, the test procedures closely followed those recommended by the EPA Committee on Methods for Toxicity Tests with aquatic organisms, except that replicate concentrations were not always used. The doses for the definitive test was selected from 5-10 geometrically equidistant concentrations and included a control. The test was conducted in 250 ml beakers containing 200 ml of the test solution and 10 daphnia. The daphnid neonates (first instar) used in the test were less than 24 hours old which were obtained by isolating gravid females for approximately 20 hours.

kanawha river water was used in the preparation of test solutions and the physico-chemical characteristics revealed the water quality to be soft and was also used for maintaing the in-houe daphnid cultures.

Dissolved oxygen and pH were determined initially and at 48 hours for all test concentrations and controls. Mortalities were recorded at 24 and 48 hours.

Under the conditions of the study, the 48-hour LC50 of n-pentyl propionate to daphnia magna was 60.0 mg/l with 95 percent confidence limits ranging between 50.6 - 71.2 mg/l.

Description of key information

In a GLP study conducted as per the methods laid out in the EPA Committee on Methods for Toxicity Tests with Aquatic Organisms, the 48-hour LC50 of pentyl propionate to Daphnia magna is 60 mg/l with 95% fiducial limits ranging between 50.6 - 71.2 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

60 mg/L

Additional information

In a GLP study conducted following the methods detailed in the EPA Committee on Methods for Toxicity Tests with Aquatic Organisms, the 48-hour LC50 of pentyl propionate to Daphnia magna was 60 mg/l with 95% fiducial limits ranging between 50.6 - 71.2 mg/l.