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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to EPA-ASTM procedures and the report contains sufficient information to permit a meaningful evaluation of study results
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study
Qualifier:
according to
Guideline:
other: EPA-ASTM guidelines
Deviations:
not specified
Principles of method if other than guideline:
not applicable
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
not applicable
Analytical monitoring:
no
Details on sampling:
no data
Vehicle:
no
Details on test solutions:
no data
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: fathead minnows
- Source: in-house
- Length at study initiation: size of the minnows ranged from 1.5-3.0 cm

ACCLIMATION
- Health during acclimation (any mortality observed): no information. Any chemical treatment required to control or prevent disease was stopped at least 5 days prior to testing
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
not applicable
Hardness:
40-60 mg/l as CaCO3
Test temperature:
no data available
pH:
no data available
Dissolved oxygen:
Minimal aeration was supplied when dissolved oxygen was below about 4 mg/l
Salinity:
not applicable
Nominal and measured concentrations:
no data
Details on test conditions:
The definitive test was conducted by EPA-ASTM procedures using 10 fathead minnows per test concentration in a total volume of 0.75 liters. Replicates were usually prepared. Temperature, fish survival, pH and dissolved oxygen levels were monitored during the test period of 96 hours. Minimal aeration was supplied when the dissolved oxygen was below about 4 mg/l.
Dechlorinated Charleston municipal water was used in tests as dilution water and in experimental controls and the same water was used for maintaining the fathead minnow population in-house. The incoming water was treated with activated carbon in two gallon filter yielding a chlorine-free dilution water with a pH very close to 7.0. Total hardness of the water measured 40-60 mg/l as CaCO3. The carbon beds were changed as needed to maintain water quality.
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
55.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 43.2-72.0 mg/l
Details on results:
No data
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
no data

None

Validity criteria fulfilled:
not specified
Conclusions:
Under the conditions of the study, the 96-hour LC50 of n-pentyl propionate to fathead minnows is 55.9 mg/l with 95% confidence limits ranging between 43.2 - 72.0 mg/l.
Executive summary:

The definitive test was conducted by EPA-ASTM procedures using 10 fathead minnows per test concentration in a total volume of 0.75 liters. Replicates were usually prepared. Temperature, fish survival, pH and dissolved oxygen levels were monitored during the test period of 96 hours. Minimal aeration was supplied when the dissolved oxygen was below about 4 mg/l. Dechlorinated Charleston municipal water was used in tests as dilution water and in experimental controls and the same water was used for maintaining the fathead minnow population in-house. The incoming water was treated with activated carbon in two gallon filter yielding a chlorine-free dilution water with a pH very close to 7.0. Total hardness of the water measured 40-60 mg/l as CaCO3. The carbon beds were changed as needed to maintain water quality.

Under the conditions of the study, the 96-hour LC50 of n-pentyl propionate to fathead minnows is 55.9 mg/l with 95% confidence limits ranging between 43.2 - 72.0 mg/l.

Description of key information

In a GLP study conducted following EPA-ASTM guidelines, the 96-hour LC50 of n-pentyl propionate was reported as 55.9 mg/l with 95% fiducial limits ranging between 43.2 - 72.0 mg/l

Key value for chemical safety assessment

LC50 for freshwater fish:
55.9 mg/L

Additional information

In a GLP study conducted following EPA-ASTM guidelines and rated a Klimisch score of 2, the 96-hour LC50 of n-pentyl propionate to fathead minnows (Pimephales promelas) was reported as 55.9 mg/l with 95% fiducial limits ranging between 43.2 - 72.0 mg/l.