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EC number: 200-926-7 | CAS number: 76-02-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- Data is from secondary source
Data source
Referenceopen allclose all
- Reference Type:
- review article or handbook
- Title:
- Dose doc no. : T230
- Author:
- RSC Publishing
- Year:
- 2 011
- Bibliographic source:
- Dictionary of Substances and their effects (DOSE):- Mutat. Res. 1983, 117(1-2), 21-30; Mutagenicity of dichloroacetylene and its degradation products trichloroacetyl chloride, trichloroacryloyl chloride and hexachlorobutadiene.; Reichert, D. et al
- Reference Type:
- other: Authoritative data base
- Title:
- HSDB Number : 6321
- Year:
- 2 011
- Bibliographic source:
- HSDB (Hazardous Substances Data Bank); Reichert D et al; Mutat Res 117 (1-2): 21-30 (1983)
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- modified Ames mutagenicity assay in vitro with the histidine-auxotrophic strains
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Trichloroacetyl chloride
- EC Number:
- 200-926-7
- EC Name:
- Trichloroacetyl chloride
- Cas Number:
- 76-02-8
- Molecular formula:
- C2Cl4O
- IUPAC Name:
- trichloroacetyl chloride
Constituent 1
Method
- Target gene:
- Histidine gene
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100
- Details on mammalian cell type (if applicable):
- histidine-auxotrophic strains
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver homogenate (S9 mix)
- Test concentrations with justification for top dose:
- NA
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- The bacteria were grown overnight in Oxoid nutrient broth, washed and resuspended in 0.1 M phosphate buffer (pH 7.4) to half the original density. The cell suspension, containing about 1 x 10 9 cells/ml, was divided into aliquots of 1.5 ml in centrifuge tubes; then 0.5 ml of either 0.1 M phosphate buffer (pH 7.4) or S9 mix and the test compound (diluted in 10/~1 acetonitrile) was added. The tubes were tightly closed with screw caps and incubated for 90 min at 37°C in a shaking water-bath. The treatment was terminated by centrifugation and resuspension in 1.1 ml fresh buffer. Aliquots of 0.5 ml cell suspension were then added to molten top agar (2 ml each) and duplicate petri dishes containing Vogel-Bonner medium E (= minimal agar) overlaid. For the determination of survival rates, an aliquot of the cell suspension was diluted by a factor of 10(4) in 0.9% NaCI; 10 µl of this dilution was added to 2 ml top agar, containing a 100-fold higher concentration of histidine (5 mM) than that in top agar for counting revertant colonies. Colonies of revertants and survivors were counted after 48 h of incubation at 37°C; the sensitivities of the tester strains were routinely checked with 2- aminoanthracene as a standard mutagen in the presence of S9 mix and with 4-nitro-o-phenylenediamine (TA98) or sodium azide (TA100) in its absence.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- True negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: other:
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The genotoxicity study on Salmonella typhimurium TA98, TA100 with and without metabolic activation gave negative result. - Executive summary:
Dichloroacetylene (DCA) is a highly reactive compound that decomposes rapidly in contact with air into a series of chlorinated aliphatic hydrocarbons (e.g., phosgene, trichloroacetyl chloride, trichloroacryloyl chloride and hexachlorobutadiene). Experiments were performed to compare the mutagenic properties of DCA and its degradation products on the histidine-dependent tester strains TA98 and TA100 of Salmonella typhimurium. In these experiments, DCA vapour was streamed under analytical control through the bacterial suspensions. DCA is soluble in aqueous solution and was stable under the experimental steady-state conditions of the bacterial exposure. There is a linear correlation between the supply of DCA vapour and solubilized DCA in the range of 1000 and 16000 ppm. Mutagenic response was observed with strain TA100 if the bacteria were suspended in Oxoid medium. No mutagenicity could be detected with strain TA98. DCA mixtures with acetylene, as used as stabilizer for animal experiments, were not mutagenic in either bacterial strain, irrespective of the presence or absence of S9 mix in the cell suspension. One of the degradation products of DCA, trichloroacryloyl chloride, showed pronounced mutagenic properties with and without drug-metabolizing enzymes. Other degradation products of DCA, such as trichloroacetyl chloride and hexachlorobutadiene, were not mutagenic, either in the presence or absence of liver homogenate.
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