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EC number: 204-352-8 | CAS number: 119-80-2
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2'-dithiodi(benzoic acid)
- EC Number:
- 204-352-8
- EC Name:
- 2,2'-dithiodi(benzoic acid)
- Cas Number:
- 119-80-2
- Molecular formula:
- C14H10O4S2
- IUPAC Name:
- 2-[(2-carboxyphenyl)disulfanyl]benzoic acid
- Test material form:
- other: Solid
- Details on test material:
- The test material had a purity of 75.7%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- -Water samples were taken from the middle of test vessels at 0, 48 and 96h
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: Upwey Trout Farm, Weymouth, Dorset, UK
ACCLIMATION
- Acclimation period: 11 days
- Type and amount of food: Promin (proprietary product)
-Pretest fasting: 24hours before dosing
-Medication: None given
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Post exposure observation period:
- -observations of mortality and toxicity symptoms made at 24, 48, 72, 96 hours
Test conditions
- Hardness:
- -measurement method: titration with EDTA using BDH total hardness indicator
- Test temperature:
- -14-16 degrees C
-measurement method: mecury in glass thermometer - pH:
- -measurement method: Corning Model 240 meter
- Dissolved oxygen:
- -measurement method: Yellow Springs Instruments dissolved oxygen meter Mmodel 51B
- Nominal and measured concentrations:
- -nominal: 180, 100, 56, 32, 18, 10mg DTBA per litre
-measured: 73, 57, 44, 28, 16, 8.1 mg DTBA per litre - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass 360mm x 230mm x 230mm 15l capacity
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Water treatment: Passed through activated carbon coarsely filtered to remove particulate material and dechlorinated with sodium thiosulphate, passed through an ultraviolet steriliser to a second set of filters of 25 and 10 microns
TEST CONDITIONS
-photoperiod: 16 hours light/8 hours dark
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 61 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 66 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 69 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 28 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- behaviour
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Results:
Measured Concentration
Cumulative Percentage Mortality
Symptoms of Toxicity Observed
24h
48h
72h
96h
24h
48h
72h
96h
73
20
60
70
80
C/abcdef
C/cefg
C/abcdefh
C/bcef
57
0
20
20
20
C/cef
C/abcefg
C/abcef
C/bcdef
44
0
0
0
0
A
C/cf
C/cef
C/bcef
28
0
0
0
0
A
A
A
A
16
0
0
0
0
A
A
A
A
8.1
0
0
0
0
A
A
A
A
Control
0
0
0
0
A
A
A
A
Key:
A=No significant effect. 10% or less of test population deead or exhibiting symptoms of toxicity
B=Partial effect. 11 -30% of test population dead or exhibiting symptoms of toxicity
C=Significant effect. 30% or more of test population dead or exhibiting symptoms of toxicity
a-quiescence
b-surfacing
c-sounding
d-loss of balance
e-dark discolouration
f-rapid respiration
g-mottled colouration
h-ceased swimming
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hour LC50 for fish = 61 mg/l. The 96 hour NOEC = 28mg/l
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