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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-dithiodi(benzoic acid)
EC Number:
204-352-8
EC Name:
2,2'-dithiodi(benzoic acid)
Cas Number:
119-80-2
Molecular formula:
C14H10O4S2
IUPAC Name:
2-[(2-carboxyphenyl)disulfanyl]benzoic acid
Test material form:
other: Solid
Details on test material:
The test material had a purity of 75.7%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
-Water samples were taken from the middle of test vessels at 0, 48 and 96h

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: Upwey Trout Farm, Weymouth, Dorset, UK

ACCLIMATION
- Acclimation period: 11 days
- Type and amount of food: Promin (proprietary product)
-Pretest fasting: 24hours before dosing
-Medication: None given

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Post exposure observation period:
-observations of mortality and toxicity symptoms made at 24, 48, 72, 96 hours

Test conditions

Hardness:
-measurement method: titration with EDTA using BDH total hardness indicator
Test temperature:
-14-16 degrees C
-measurement method: mecury in glass thermometer
pH:
-measurement method: Corning Model 240 meter
Dissolved oxygen:
-measurement method: Yellow Springs Instruments dissolved oxygen meter Mmodel 51B
Nominal and measured concentrations:
-nominal: 180, 100, 56, 32, 18, 10mg DTBA per litre
-measured: 73, 57, 44, 28, 16, 8.1 mg DTBA per litre
Details on test conditions:
TEST SYSTEM
- Test vessel: glass 360mm x 230mm x 230mm 15l capacity

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Water treatment: Passed through activated carbon coarsely filtered to remove particulate material and dechlorinated with sodium thiosulphate, passed through an ultraviolet steriliser to a second set of filters of 25 and 10 microns

TEST CONDITIONS
-photoperiod: 16 hours light/8 hours dark

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
61 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
66 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
69 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
28 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
behaviour

Any other information on results incl. tables

Sublethal observations / clinical signs:

Results:

Measured Concentration

Cumulative Percentage Mortality

Symptoms of Toxicity Observed

24h

48h

72h

96h

24h

48h

72h

96h

73

20

60

70

80

C/abcdef

C/cefg

C/abcdefh

C/bcef

57

0

20

20

20

C/cef

C/abcefg

C/abcef

C/bcdef

44

0

0

0

0

A

C/cf

C/cef

C/bcef

28

0

0

0

0

A

A

A

A

16

0

0

0

0

A

A

A

A

8.1

0

0

0

0

A

A

A

A

Control

0

0

0

0

A

A

A

A

Key:

A=No significant effect. 10% or less of test population deead or exhibiting symptoms of toxicity

B=Partial effect. 11 -30% of test population dead or exhibiting symptoms of toxicity

C=Significant effect. 30% or more of test population dead or exhibiting symptoms of toxicity

a-quiescence

b-surfacing

c-sounding

d-loss of balance

e-dark discolouration

f-rapid respiration

g-mottled colouration

h-ceased swimming

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96 hour LC50 for fish = 61 mg/l. The 96 hour NOEC = 28mg/l