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EC number: 221-140-0 | CAS number: 3010-96-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2,4,4-tetramethylcyclobutane-1,3-diol, mixed isomers
- EC Number:
- 221-140-0
- EC Name:
- 2,2,4,4-tetramethylcyclobutane-1,3-diol, mixed isomers
- Cas Number:
- 3010-96-6
- Molecular formula:
- C8H16O2
- IUPAC Name:
- 2,2,4,4-tetramethylcyclobutane-1,3-diol
- Details on test material:
- - Name of test material (as cited in study report): PM 1265 (internal product designation) and Cyclobutanediol, 2,2,4,4-Tetramethyl
- Substance type: production sample
- Physical state: solid
- Lot/batch No.:TS060505
- Storage condition of test material: Ambient conditions
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the test solutions were collected at approximately 0 and 96 hours to measure concentrations of the test substance. Samples at test initiation were collected from the individual batches of test solution prepared for each treatment and control group prior to distribution into the test chambers. At exposure termination, samples were collected from the pooled replicates from each treatment and control group. All samples were collect in glass vials and processed immediately for analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A primary stock solution was prepared by dissolving PM-1265 in freshwater algal medium at a nominal concentration of 120 mg/L. The stock was inverted at least 20 times to mix and appeared clear and colorless. The primary stock was proportionally diluted with freshwater algal medium to prepare the four additional test solutions at nominal concentrations of 7.5, 15, 30 and 60 mg/L. All test solutions appeared clear and colorless.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Original algal cultures of Pseudokirchneriella subcapitata (UTCC 37) were obtained from the University of Toronto, and had been maintained in culture medium at Wildlife International, Ltd., Easton, Maryland. Algal cells used in this test were obtained from Wildlife International, Ltd. cultures that had been actively growing in culture medium for at least two weeks prior to test initiation. The culture was last transferred to fresh medium four days prior to test initiation.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 24 ± 2°C
- pH:
- The pH of the test solutions at test initiation was 7.8 and at exposure termination ranged from 9.2 to 9.4. The pH tended to increase relative to increases in algal densities, which is typical for tests conducted with Pseudokirchneriella subcapitata.
- Nominal and measured concentrations:
- Nominal concentrations selected for use in this study were 7.5, 15, 30, 60 and 120 mg/L.
The mean measured test concentrations were 7.3, 13, 25, 51 and 108 mg/L - Details on test conditions:
- Prior to test initiation, an inoculum of the algal cells was prepared in freshwater algal medium at a concentration of approximately 1.0 X 10e6 cells/mL. The concentration of algal cells was verified using a hemacytometer and microscope, and 1.0 mL of the inoculum was added to each test chamber to achieve a nominal concentration of approximately 10,000 cells/mL at test initiation. Test chambers were sterile, 250-mL Erlenmeyer flasks plugged with foam stoppers, and contained 100 mL of test or control medium. The algae were held under 24 hours per day of cool-white fluorescent lighting at an intensity of 4300± 10 % lux. Test medium samples were collected from each replicate of the treatment and control group for the determination of algal cell densities. Samples were collected at approximately 24-hour intervals during the 96-hour exposure. Cell counts were performed using an electronic particle counter.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 108 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 108 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 108 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 108 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
A 96-Hour Toxicity Test with the Freshwater Alga (Pseudokirchneriella subcapitata) was conducted on PM 1265. The 72 and 96-hour EC50, EbC50 and ErC50 values, based on cell density, area under the growth curve and growth rate, respectively, for Pseudokirchneriella subcapitata exposed to PM-1265 were >108 mg/L, the highest measured concentration tested. The 72 and 96-hour NOAEC for each growth parameter was 108 mg/L.
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