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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 100 mg test substance and 10 mg Arkopal were dissolved in 1 ml Dioxane and made up to 1000 ml with water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Arkopal and Dioxane
- Highest vehicle concentration 1034 mg/L Dioxane and 10 mg/L Arkopal
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Age at study initiation: 0 - 24 h
- Feeding during test: none

BREEDING
- Type and amount of food: with suspension of green algae and yeast
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Test temperature:
20 - 21 °C
pH:
7.7 - 7.9
Dissolved oxygen:
91 - 96% saturation
Nominal and measured concentrations:
Nominal test concentrations: control, 10, 18, 32, 58, 100 mg/L
Mean measured test concentrations: -, 8.3, 13.5, 18.3, 28.3, 63.7 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 ml test tubes filled with 10 ml solution covered with steel caps
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Intervals of water quality measurement: (pH, 02 content, temperature at the beginning and at the end of the test

OTHER TEST CONDITIONS
- Photoperiod: 16 hours dai1y
- Light intensity: Fluorescent 1ight

EFFECT PARAMETERS MEASURED: mobility after 24 h
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
8.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
15.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 11.5 - 18.1 mg/L
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 63.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
not specified
Conclusions:
The 24 hour EC50 of the test material to Daphnia magna was determined to be 15.3 mg/l under the conditions of the test.

Description of key information

The study provided was conducted to recognised testing guidelines and with GLP certification.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
15.3 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna was tested in a static test system performed according to OECD guideline 202. At test termination after 24 hours an EC50 of 15.3 mg/L (nominal) was determined (Ciba-Geigy, 1983c). However, emulsifiers in concentration above 100 mg/L have been used.