2-methyl-1-(4-methylthiophenyl)-2-morpholinopropan-1-one

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 100 mg test substance and 10 mg Arkopal were dissolved in 1 ml Dioxane and made up to 1000 ml with water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Arkopal and Dioxane
- Highest vehicle concentration 1034 mg/L Dioxane and 10 mg/L Arkopal

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation: 0 - 24 h
- Feeding during test: none

BREEDING
- Type and amount of food: with suspension of green algae and yeast

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test temperature:

20 - 21 °C

pH:

7.7 - 7.9

Dissolved oxygen:

91 - 96% saturation

Nominal and measured concentrations:

Nominal test concentrations: control, 10, 18, 32, 58, 100 mg/L
Mean measured test concentrations: -, 8.3, 13.5, 18.3, 28.3, 63.7 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 ml test tubes filled with 10 ml solution covered with steel caps
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Intervals of water quality measurement: (pH, 02 content, temperature at the beginning and at the end of the test

OTHER TEST CONDITIONS
- Photoperiod: 16 hours dai1y
- Light intensity: Fluorescent 1ight

EFFECT PARAMETERS MEASURED: mobility after 24 h

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

8.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

15.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 11.5 - 18.1 mg/L

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 63.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

The 24 hour EC50 of the test material to Daphnia magna was determined to be 15.3 mg/l under the conditions of the test.

Description of key information

The study provided was conducted to recognised testing guidelines and with GLP certification.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

15.3 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna was tested in a static test system performed according to OECD guideline 202. At test termination after 24 hours an EC50 of 15.3 mg/L (nominal) was determined (Ciba-Geigy, 1983c). However, emulsifiers in concentration above 100 mg/L have been used.