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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
according to
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
not specified
GLP compliance:
not specified
Oxygen conditions:
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Bacteria collected from activated sludge of the sewage treatment plant of CH-4310 Rheinfelden on 13/06/1983. The preparation of the inoculum was carried out according to the method described in the guideline.
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
- Test vessel: 2 L flasks equipped with gas inlet and magnetic stirrer.
- Test water: The test medium was prepared according to the method described in the guideline.
- Temperature: 22 ± 2°C
- Duration: 28 days
- Air: approx, 50 ml/min , free of carbondioxide.
Reference substance:
% degradation (CO2 evolution)
<= 1
Sampling time:
28 d
Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
No degradation of the test material was observed under the conditions of the test.

Description of key information

The study provided was conducted to recognised testing guidelines and with GLP certification.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:

Additional information

The biodegradability of the test substance was investigated in a study according to GLP and OECD guideline 301 B. After 28 days a biodegradation rate < 1% was measured (Ciba-Geigy, 1983b).