7-aminonaphthalene-1,3,5-trisulphonic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted following OECD guideline, but report does not mention whether GLP was followed and sufficient data is available for interpretation of results.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

OECD guideline 202 followed.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

7-aminonaphthalene-1,3,5-trisulphonic acid

EC Number:

248-394-5

EC Name:

7-aminonaphthalene-1,3,5-trisulphonic acid

Cas Number:

27310-25-4

Molecular formula:

C10H9NO9S3

IUPAC Name:

7-aminonaphthalene-1,3,5-trisulfonic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): FAT 93527/A
- Physical state: Powder
- Analytical purity: ca. 50%
- Lot/batch No.: Lot 0031026A0
- Expiration date of the lot/batch: January 10, 2006
- Storage condition of test material: room temperature
- Solubility: ca.120g/L

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
None

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

None

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

Stock solution: 100.0 mg test item were mixed with 200 mL test medium in a volumetric flask, made up to 1000 mL with test water.

Test concentrations: A range-finding test with the following test concentrations was carried out: 0.01, 0.1, 1.0, 10 and 100 mg/L No Immobilisation of Daphnia were observed at the highest concentration, hence the test concentrations for the definitive test were chosen as: 4.3, 9.4, 21, 45 and 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: Solvias AG, Switzerland
- Age at study initiation (mean and range, SD): 24 hr
- Method of breeding: The Daphnia were bred in the laboratories of the GLP Test Facility Solvias under standardised conditions.

BREEDING:
Cultures of Daphnia magna are held in glass vessels containing approximately 3 litres of reconstituted water at 18 -22°C. The water is renewed partially three times per
week. Daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplementpd by a Tetramin-extract in such quantities that the food is consumed within 24 hours.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

None

Post exposure observation period:

Study terminated after 48 h.

Test conditions

Hardness:

178mg CaCO3/L

Test temperature:

20.2 - 20.8 Deg C

pH:

8.1

Dissolved oxygen:

96 - 97%

Salinity:

No data

Nominal and measured concentrations:

4.3, 9.4 and 21, 45, and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 ml beaker
- Type (delete if not applicable): open
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 50-100 ml test medium with 10 animals per beaker

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
NaHCO3: 65mg/L
MgSO4*7H2O: 123.3mg/L
KCl: 5.8mg/L
CaCI2 *2H2O: 294mg/L
Na2SiO3: 4.3mg/L
NaNO3: 274 micro g/L
KH2PO4: 143 micro g/L
K2HPO4: 184 micro g/L


- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4 : 1
- Na/K ratio: 10 : 1


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light daily, 8 hours dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS:
- Range finding study: 0.01, 0.1, 1.0, 10 and 100 mg/L
- Test concentrations: 4.3, 9.4, 21, 45 and 100 mg/L
- Results used to determine the conditions for the definitive study: Immobilisation

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: No mortality

Results with reference substance (positive control):

- EC50/LC50: The EC50 for potassium dichromate after 24 hours of exposure was determined among 46 laboratories in an international ring-test with Daphnia magna and found to be at 1.5 mg/L (0.9 to 1.9 mg/L).
Therefore, the cultures of Daphnia magna used by the test facility were demonstrated to be adequately sensitive.

Reported statistics and error estimates:

No statistics used.

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 value after 48 hours was determined to be greater than 100 mg/L
The EC100 value after 48 hours was determined to be greater than 100 mg/L

Executive summary:

FAT 93527/A was tested for its acute toxicity to Daphnia magna in a 48 hour static test following OECD test guideline 202.

 

The nominal concentrations of FAT 93527/A tested were: 4.3, 9.4, 21, 45 and 100 test item/L. Additionally, a blank control without test item was carried out. Test concentrations were prepared from a stock solution.

No Daphnia were immobilised in the control and test concentrations of 4.3, 9.4 and 21, 45, and 100 mg/L after an exposure period of 48h.

 

The EC50 value after 48 hours was determined to be greater than 100 mg/L

The EC100 value after 48 hours was determined to be greater than 100 mg/L.

 

According to Directive 93/21/EEC (adaptation to Directive 67/548/EEC) the ecotoxicological classification of FAT93527/A is 'not toxic' to Daphnia magna following an exposure period of 48 hours.