Trisodium 6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Follows a recognnised guideline and conforms to GLP standards.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate

IUPAC Name:

trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Range-finding test: trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate was dissolved directly into reconstituted water. 100mg was added to reconstituted water and the volume adjusted to 1 litre to give the 100 mg/l test concentration. This was serially diluted to give the remaining conconcentrations of 10, 1.0 and 0.10 mg/l.

Definitive test: trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate was dissolved directly into reconstituted water. 100mg was added to reconstituted water and the volume adjusted to 1 litre to give the 100 mg/l test concentration.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: In-house laboratory culture
- Age at study initiation (mean and range, SD): < 24 hours old
- Method of breeding: Parthenogenesis
- Feeding during test
- Food type: Suspension of algae
- Frequency: Daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

22 C

pH:

7.7 (8.0 for control)

Dissolved oxygen:

8.5 mg O2/l

Nominal and measured concentrations:

Range-finding test nominal concentrations: 0.10, 1.0, 10, 100 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 ml glass jars containing 200 ml of test preparation.
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): Not renewed
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours dark


TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.10, 1.0, 10, 100 mg/l (nominal)
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Range-finding test: No immobilisation was observed at any test concentration throughout the duration of the test.
Definitive test: There was no immobilisation in 20 daphnids exposed to a test concentration of 100 mg/l for a period of 48 hours.

Results with reference substance (positive control):

The results from the positive control with potassium dichromate were within the normal range for this reference material (see table 1). The mean 48-hour EC50 value calculated from all positive controls was 0.78 mg/l.

Any other information on results incl. tables

Table 1. Positive control results

Time (h)	EC50 (mg/l)	95% Confidence limits (mg/l)
3	> 3.2	-
24	0.97	0.85 - 1.1
48	0.70	0.62 - 0.80

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-hour EC50 of greater than 100 mg/l. Correspondingly the NOEC was 100 mg/l.

Executive summary:

The 48h-acute toxicity of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate to Daphnia magna was studied under static conditions.  Daphnids were exposed to control and test chemical at a nominal concentration of 100 mg a.i./L for 48 -hr. Immobilization and sublethal effects were observed daily.  The 48h EC₅₀was >100 mg a.i./L.  The 48h NOEC based on immobilization was 100 mg a.i./L. 

 

Based on the results of this study, trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate is not classified in accordance with the criteria of the GHS classification system.

 

 

Results Synopsis

 

Test Organism Age (e.g. 1^stinstar): < 24 hours old

Test Type (Flowthrough, Static, Static Renewal): static

 

NOEL: 100 mg a.i./L

EC₅₀:  >100 mg a.i./L