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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Follows a recognnised guideline and conforms to GLP standards.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report Date:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Range-finding test: trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate was dissolved directly into reconstituted water. 100mg was added to reconstituted water and the volume adjusted to 1 litre to give the 100 mg/l test concentration. This was serially diluted to give the remaining conconcentrations of 10, 1.0 and 0.10 mg/l.

Definitive test: trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate was dissolved directly into reconstituted water. 100mg was added to reconstituted water and the volume adjusted to 1 litre to give the 100 mg/l test concentration.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: In-house laboratory culture
- Age at study initiation (mean and range, SD): < 24 hours old
- Method of breeding: Parthenogenesis
- Feeding during test
- Food type: Suspension of algae
- Frequency: Daily

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
22 C
pH:
7.7 (8.0 for control)
Dissolved oxygen:
8.5 mg O2/l
Nominal and measured concentrations:
Range-finding test nominal concentrations: 0.10, 1.0, 10, 100 mg/l.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 ml glass jars containing 200 ml of test preparation.
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): Not renewed
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours dark


TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.10, 1.0, 10, 100 mg/l (nominal)
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Range-finding test: No immobilisation was observed at any test concentration throughout the duration of the test.
Definitive test: There was no immobilisation in 20 daphnids exposed to a test concentration of 100 mg/l for a period of 48 hours.
Results with reference substance (positive control):
The results from the positive control with potassium dichromate were within the normal range for this reference material (see table 1). The mean 48-hour EC50 value calculated from all positive controls was 0.78 mg/l.

Any other information on results incl. tables

Table 1. Positive control results

 Time (h) EC50 (mg/l)  95% Confidence limits (mg/l) 
> 3.2 
24  0.97  0.85 - 1.1 
48  0.70  0.62 - 0.80 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-hour EC50 of greater than 100 mg/l. Correspondingly the NOEC was 100 mg/l.
Executive summary:

The 48h-acute toxicity of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate to Daphnia magna was studied under static conditions.  Daphnids were exposed to control and test chemical at a nominal concentration of 100 mg a.i./L for 48 -hr. Immobilization and sublethal effects were observed daily.  The 48h EC50was >100 mg a.i./L.  The 48h NOEC based on immobilization was 100 mg a.i./L. 

 

Based on the results of this study, trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate is not classified in accordance with the criteria of the GHS classification system.

 

 

Results Synopsis

 

Test Organism Age (e.g. 1stinstar): < 24 hours old

Test Type (Flowthrough, Static, Static Renewal): static

 

NOEL: 100 mg a.i./L

EC50:  >100 mg a.i./L