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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
deviation is conidered as uncritical
Principles of method if other than guideline:
Temperature in the limit test lay in the range of 21.7 - 22.5 °C instead of 18 - 22 °C. As the difference is only marginal and respiration rate of the activated sludge lay in the normal range, the deviation is considered as uncritical.
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
activated sludge, domestic
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenole
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Conclusions:
NOEC = 1,000 mg/L
EC50 >> 1,000 mg/L
Executive summary:

In the limit test, the mean inhibition which was caused by the test item was 12 %. Therefore, no main study was performed and no exact 3h EC50 was determined. The value is stated > 1,000 mg/L". As inhibition of the treatment 1,000 mg/L lies below the recommended limit for variation within the controls, 1,000 mg/L can be stated as NOEC.

All validity criteria were met.

Description of key information

NOEC(3 h) = 1000 mg/L	
EC20(3 h) > 1000 mg/L
EC50(3 h) > 1000 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L
EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information