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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22.11.2004 to 09.12.2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
OS Menthol Ester
IUPAC Name:
OS Menthol Ester
Constituent 2
Chemical structure
Reference substance name:
(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl (2R,5R)-5-hydroxy-1,3-oxathiolane-2-carboxylate
EC Number:
604-569-1
Cas Number:
147126-62-3
Molecular formula:
C14H24O4S
IUPAC Name:
(1R,2S,5R)-2-isopropyl-5-methylcyclohexyl (2R,5R)-5-hydroxy-1,3-oxathiolane-2-carboxylate
Test material form:
solid: compact

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Temperature on receipt was 13.0°C. The maximum allowable temperature is 10°C according to Canadian regulations or guidance documents.
Samples submitted to the laboratory soon after sampling are exempt. provided that cooling has been initiated. Cooling is not required for certain situations such as: Waste for classification or specific matrices or tests such as PCB in oil. There are no other notable comments.

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout (Oncorhynchus mykiss)
- Length at study initiation (mean length): 3.38 cm
- Feeding during test: no
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test conditions
- Type and amount of food: No. 1 pellet size trout food at a daily ration approximating 1-4% wet body weight
- Feeding frequency: 7 times a week
- Health during acclimation (any mortality observed): 5, 5% mortatity in 7 days prior to test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
hardness - 260 mg/Las CaC03
Nominal and measured concentrations:
nominal: 220, 100, 46, 22, 10, 4.6 mg/L
(water-accommodated fraction)
Details on test conditions:
Details on test conditions
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass test aquaria of 10 L volume filled with Y kg test medium
- Aeration: oil free compressed air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1 (1)
- No. of vessels per control (replicates): 1 (1)
- Biomass loading rate: 0,3 g fish/L at maximum

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Well water
- Total organic carbon: 260 mg/L
- Alkalinity: 236 mg/L
- Ca/Mg ratio: 61.4 mg Ca/L, 29.1 mg Mg/L
- Conductivity: 522 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: The study plan indicated that water quality parameters (pH, dissolved oxygen, and conductivity) would be measured daily.

OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle was 16 to 8 h

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality and abnormal behaviour (e.g., erratic swimming) were recorded at 3, 24, 48, 72 and 96-hours.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
67.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
36.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
34 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

 Nominal conc. of test substance (mg/L)              Number of dead fish
               exposure period
   3h  24h  48h  72h  96h
 0 (Control)  0  0  0  0  0
 220  0 10  10  10  10
 100  0 10  10  10  10
 46  0  0  8  8  9
 22  0  0  0  0  0
 10  0  0  0  0  0
 4.6  0  0  0  0  0

Applicant's summary and conclusion

Conclusions:
This ecotoxicological evaluation resulted in a 96-hour LC50 of 34.4 mg/L for rainbow trout, based on nominal concentrations of OS MENTHOL ESTER.

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