Registration Dossier
Registration Dossier
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EC number: 604-569-1 | CAS number: 147126-62-3
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22.11.2004 to 09.12.2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- OS Menthol Ester
- IUPAC Name:
- OS Menthol Ester
- Reference substance name:
- (1R,2S,5R)-2-isopropyl-5-methylcyclohexyl (2R,5R)-5-hydroxy-1,3-oxathiolane-2-carboxylate
- EC Number:
- 604-569-1
- Cas Number:
- 147126-62-3
- Molecular formula:
- C14H24O4S
- IUPAC Name:
- (1R,2S,5R)-2-isopropyl-5-methylcyclohexyl (2R,5R)-5-hydroxy-1,3-oxathiolane-2-carboxylate
- Test material form:
- solid: compact
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Temperature on receipt was 13.0°C. The maximum allowable temperature is 10°C according to Canadian regulations or guidance documents.
Samples submitted to the laboratory soon after sampling are exempt. provided that cooling has been initiated. Cooling is not required for certain situations such as: Waste for classification or specific matrices or tests such as PCB in oil. There are no other notable comments.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout (Oncorhynchus mykiss)
- Length at study initiation (mean length): 3.38 cm
- Feeding during test: no
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test conditions
- Type and amount of food: No. 1 pellet size trout food at a daily ration approximating 1-4% wet body weight
- Feeding frequency: 7 times a week
- Health during acclimation (any mortality observed): 5, 5% mortatity in 7 days prior to test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- hardness - 260 mg/Las CaC03
- Nominal and measured concentrations:
- nominal: 220, 100, 46, 22, 10, 4.6 mg/L
(water-accommodated fraction) - Details on test conditions:
- Details on test conditions
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass test aquaria of 10 L volume filled with Y kg test medium
- Aeration: oil free compressed air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1 (1)
- No. of vessels per control (replicates): 1 (1)
- Biomass loading rate: 0,3 g fish/L at maximum
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Well water
- Total organic carbon: 260 mg/L
- Alkalinity: 236 mg/L
- Ca/Mg ratio: 61.4 mg Ca/L, 29.1 mg Mg/L
- Conductivity: 522 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: The study plan indicated that water quality parameters (pH, dissolved oxygen, and conductivity) would be measured daily.
OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle was 16 to 8 h
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality and abnormal behaviour (e.g., erratic swimming) were recorded at 3, 24, 48, 72 and 96-hours.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 67.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 36.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 34 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Nominal conc. of test substance (mg/L) Number of dead fish exposure period 3h 24h 48h 72h 96h 0 (Control) 0 0 0 0 0 220 0 10 10 10 10 100 0 10 10 10 10 46 0 0 8 8 9 22 0 0 0 0 0 10 0 0 0 0 0 4.6 0 0 0 0 0
Applicant's summary and conclusion
- Conclusions:
- This ecotoxicological evaluation resulted in a 96-hour LC50 of 34.4 mg/L for rainbow trout, based on nominal concentrations of OS MENTHOL ESTER.
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