Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
28 d

Results and discussion

% Degradation
Parameter:
% degradation (TOC removal)
Value:
>= 12
Sampling time:
14 d
Details on results:
TOC analysis and theoretical C02 production
The TOC concentration of the 1g NA-872 AMINOACETAT/l solution was 642.4 ± 37.07 ppm.
Based on the TOC concentration the Theoretical CO2 production (ThC02) of the NA-872 AMINOACETAT solution was calculated to be
2.36 mg C02/ml.
The concentration was 37 ml of 1g NA-872 AMINOACETAT/l (A and B) in 2 litres test medium. Hence, the theoretical C02 production following
complete degradation was 87.3 mg per 2 litres for A and B.
The positive control contained 80.3 mg sodium acetate (ThC02= 1.07 mg e02/mg) resulting in a theoretical C02 production following complete
degradation of 85.9 mg per 2 litres.
The toxicity control contained 80.3 mg sodium acetate and 37 ml of 1g NA-872 AMINOACETAT/l in 2 litres of test medium. Hence, the theoretical
C02 production following complete degradation of NA-872 AMINOACETAT plus sodium acetate was 173.2 mg per 2 litres.
The relative degradation values calculated from the measurements performed during the test period revealed 12% degradation of
NA-872 AMINOACETAT in test bottle A and no significant degradation in test bottle B.
Thus, the criterion for ready biodegradability (at least 60% degradation within 10 days of biodegradation exceeding 10%) was not met.
In the toxicity control more than 25% degradation occurred within 14 days (based on ThC02)' Therefore, the test substance was assumed to be not
inhibitory.

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
In conclusion, NA-872 AMINOACETAT was not readily biodegradable under the conditions of the modified Sturm test presently performed.
Executive summary:

NA-872 AMINOACETAT was tested for its ready biodegradability in the carbon dioxide (C02) evolution test (modified Sturm test). The study procedure was based on EEC directive 92/69, C.4-C, December 1992, and OECD guideline No. 301 B July 17, 1992. Since calculation of the theoretical TOC value was not possible this value was determined by analysis. Prior to the biodegradation study the TOC concentration of a 1 g NA-872 AMINOACETAT/l solution in milli-Q water was determined. The TOC concentration of the 1 g NA-872 AMINOACETAT/l solution was 642.4 ± 37.07 ppm. Based on the TOC concentration the Theoretical C02 production (ThC02) of the NA-872 AMINOACETAT solution was calculated to be 2.36 mg C02 /ml. In the modified Sturm test 37 ml of the 1 g NA-872 AMINOACETAT/l solution was tested in 2 litres test medium, corresponding to 12 mg TOC/1. The relative degradation values calculated from the measurements performed during the test period revealed 12% degradation of NA-872 AMINOACETAT in test bottle A and no significant degradation in test bottle B. Thus, the criterion for ready biodegradability (at least 60% degradation within 10 days of biodegradation exceeding 10%) was not met. In the toxicity control NA-872 AMINOACETAT was found to be not inhibitory. Since all acceptability criteria prescribed by the protocol were met, this study was considered to be valid. In conclusion, NA-872 AMINOACETAT was not readily biodegradable under the conditions of the modified Sturm test presently performed.