4-[[(2-aminophenyl)methyl]amino]cyclohexyl acetate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-[[(2-aminophenyl)methyl]amino]cyclohexyl acetate

EC Number:

298-842-9

EC Name:

4-[[(2-aminophenyl)methyl]amino]cyclohexyl acetate

Cas Number:

93839-71-5

Molecular formula:

C13H20N2O.C2H4O2

IUPAC Name:

4-[[(2-aminophenyl)methyl]amino]cyclohexyl acetate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Results and discussion

% Degradation

Parameter:

% degradation (TOC removal)

Value:

>= 12

Sampling time:

14 d

Details on results:

TOC analysis and theoretical C02 production
The TOC concentration of the 1g NA-872 AMINOACETAT/l solution was 642.4 ± 37.07 ppm.
Based on the TOC concentration the Theoretical CO2 production (ThC02) of the NA-872 AMINOACETAT solution was calculated to be
2.36 mg C02/ml.
The concentration was 37 ml of 1g NA-872 AMINOACETAT/l (A and B) in 2 litres test medium. Hence, the theoretical C02 production following
complete degradation was 87.3 mg per 2 litres for A and B.
The positive control contained 80.3 mg sodium acetate (ThC02= 1.07 mg e02/mg) resulting in a theoretical C02 production following complete
degradation of 85.9 mg per 2 litres.
The toxicity control contained 80.3 mg sodium acetate and 37 ml of 1g NA-872 AMINOACETAT/l in 2 litres of test medium. Hence, the theoretical
C02 production following complete degradation of NA-872 AMINOACETAT plus sodium acetate was 173.2 mg per 2 litres.
The relative degradation values calculated from the measurements performed during the test period revealed 12% degradation of
NA-872 AMINOACETAT in test bottle A and no significant degradation in test bottle B.
Thus, the criterion for ready biodegradability (at least 60% degradation within 10 days of biodegradation exceeding 10%) was not met.
In the toxicity control more than 25% degradation occurred within 14 days (based on ThC02)' Therefore, the test substance was assumed to be not
inhibitory.

Applicant's summary and conclusion

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

In conclusion, NA-872 AMINOACETAT was not readily biodegradable under the conditions of the modified Sturm test presently performed.

Executive summary:

NA-872 AMINOACETAT was tested for its ready biodegradability in the carbon dioxide (C02) evolution test (modified Sturm test). The study procedure was based on EEC directive 92/69, C.4-C, December 1992, and OECD guideline No. 301 B July 17, 1992. Since calculation of the theoretical TOC value was not possible this value was determined by analysis. Prior to the biodegradation study the TOC concentration of a 1 g NA-872 AMINOACETAT/l solution in milli-Q water was determined. The TOC concentration of the 1 g NA-872 AMINOACETAT/l solution was 642.4 ± 37.07 ppm. Based on the TOC concentration the Theoretical C02 production (ThC02) of the NA-872 AMINOACETAT solution was calculated to be 2.36 mg C02 /ml. In the modified Sturm test 37 ml of the 1 g NA-872 AMINOACETAT/l solution was tested in 2 litres test medium, corresponding to 12 mg TOC/1. The relative degradation values calculated from the measurements performed during the test period revealed 12% degradation of NA-872 AMINOACETAT in test bottle A and no significant degradation in test bottle B. Thus, the criterion for ready biodegradability (at least 60% degradation within 10 days of biodegradation exceeding 10%) was not met. In the toxicity control NA-872 AMINOACETAT was found to be not inhibitory. Since all acceptability criteria prescribed by the protocol were met, this study was considered to be valid. In conclusion, NA-872 AMINOACETAT was not readily biodegradable under the conditions of the modified Sturm test presently performed.