1,1-dimethoxytrimethylamine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: OECD guideline study conducted in accordance to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1,1-dimethoxytrimethylamine

EC Number:

225-063-3

EC Name:

1,1-dimethoxytrimethylamine

Cas Number:

4637-24-5

Molecular formula:

C5H13NO2

IUPAC Name:

1,1-dimethoxy-N,N-dimethylmethanamine

Details on test material:

TS-Freetext:
Dimethylformamiddimethylacetal , purity 95.9%

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

S-9 mix

Test concentrations with justification for top dose:

20 - 5000 ug/plate

Details on test system and experimental conditions:

IUCLID4 Type: Ames test

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

According to the results of the present study, the test substance 1,1-dimethoxytrimthylamine is not mutagenic in the Ames test and Escherichia coli reverse mutation assay under the experimental conditions chosen here.

Executive summary:

The substance 1,1-dimethoxytrimthylamine was tested for mutagenicity in the Ames test and in the E. coli reverse mutation assay both in the standard plate test and in the preincubation test with and without the addition of a metabolizing system obtained from rat liver (S-9 mix) using the Salmonella strains TA 1535, TA 100, TA 1537, TA 96 and Escherichia coli WP12 uvrA. No increase in the number of revertants was observed either with or without metabolic activation. Furthermore, no bacteriotoxic effect was observed.