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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
From 01 MAR 2005 to 02 MAR 2005
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
GLP according to German Chemikaliengesetzt and Directive 88/320/EEC

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
total hardness of 160 to 180 mg CaCO3/L
Test temperature:
18 - 22 °C, ± 1 °C constant
pH:
Control: 7.8 - 7.94
Treatment: 7.57 - 7.81
Dissolved oxygen:
[mg/L]
Control: 7.67 - 8.69
Treatment: 8.05 - 8.69
Nominal and measured concentrations:
Nominal limit concentation: 100 mg/L
Control: 0 mg/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: As the test item is highly insoluble in water, a saturated solution was prepared at the nominal concentration and filtrated
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: As the test item is highly insoluble in water, a saturated solution was prepared at the nominal concentration and filtrated
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: As the test item is highly insoluble in water, a saturated solution was prepared at the nominal concentration and filtrated
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: As the test item is highly insoluble in water, a saturated solution was prepared at the nominal concentration and filtrated
Reported statistics and error estimates:
The effects of the limit concentration were determined directly from the test results.
EC50- and EC10-values were only calculated for the reference item via probit analysis according to WEBER (1986). Calculation of the confidence intervals was carried out using standard procedures according to BREITIG G. & TÜMPLING W. (1982).

Software
All data were computer generated and rounded for presentation from the full derived data. Consequently, if calculated manually based on the given data minor variations may occur from these figures. Calculations were carried out using software: SigmaPlot rel. 2000 (2000), SPSS CORPORATION

Applicant's summary and conclusion

Validity criteria fulfilled:
yes