Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Study initiation date: February 15, 1995 to study completion date: May 30, 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
Deviations:
yes
Remarks:
observation period for 21 days instead of 28 days
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: solid
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 400047.32
- Expiration date of the lot/batch: December 1998

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/vehicle: <0.1 g/l [in water]

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic (adaptation not specified)
Details on inoculum:
Strain / Inoculation: Mixture of polyvalent bacteria collected on February 21, 1995 in the aeration tank of a domestic sewage treatment plant, PRO RHENO Basle.
Bacteria concentration: 100 mg/l suspended solids
Duration of test (contact time):
21 d
Initial test substance concentration
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
Experimental conditions
Water / Nutrient: According to the specification of the test method
Temperature: 25 ± 2 °C
Test duration: 21 days
Lighting: none
Estimation Technique: Voith Sapromat; 02-consumption automatically/manually measured in milligrams oxygen per liter.

Test concentrations:
Test substance: 30 mg/l (added without any pretreatment);
Reference substance: 100 mg/l (added without any pretreatment)
Reference substance
Reference substance:
other: Natriumbenzoat powder

Results and discussion

Preliminary study:
No data
% Degradation
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
21 d
Details on results:
Test substance:
The biodegradation of FAT 21016/C after 21 days corrected by the inoculum control and measured as BOD versus ThOD is 0 %

Abiotic control:
The abiotic degradation at the end of the test and measured as BOD versus ThOD is 0 %

BOD5 / COD results

Results with reference substance:
Reference substance:
The biodegradation of the reference substance corrected by the inoculum control and measured as BOD versus ThOD is
77 % after 7 days
and is 75 % after 14 days
and is 72 % after 21 days

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not inherently biodegradable
Conclusions:
The biodegradation of the test substance FAT 21016/C after 21 days was 0 %
Executive summary:

The inherent biodegradability of FAT 21016/C was determined in a 21 days MITI (II) - Test according to the OECD Guideline for Testing of Chemicals, No. 302C. This study was performed in compliance with Good Laboratory Practice (GLP) in Switzerland, Procedures and Principles, March 1986, issued by the Federal Department of the Interior and the Intercantonal Office for the Control of Medicaments, Switzerland.

The test substance was tested in a concentration of 30 mg/l. The reference substance (Natriumbenzoat) was tested in a concentration of 100 mg/l. The oxygen consumption was automatically measured by Voith Sapromat. The selected measuring range was X1, (250 ml testmedium per flask) . The oxygen consumption was given in milligrams O2 per liter.

Biodegradation after 21 days of the test substance of FAT 21016/C is 0 %

Biodegradation after 21 days of the reference substance is 72 %

Hence, FAT 21016/C is not biodegradable.