Silicic acid, titanium salt

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

no data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, sufficiently documented, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Principles of method if other than guideline:

Mutations were quantified by counting anaphase aberrations. Negative (0.85 %) and positive (0.1 µg/l triethylene melamine) controls were run in parallel.

GLP compliance:

no

Type of assay:

in vitro mammalian chromosome aberration test

Test material

Test material information

Constituent 1

Reference substance name:

112926-00-8

EC Number:

601-214-2

Cas Number:

112926-00-8

IUPAC Name:

112926-00-8

Details on test material:

- Name of test material (as cited in study report): Syloid 244
- Physical state: fine white powder

Method

Species / strain

Species / strain / cell type:

mammalian cell line, other: human embryonic lung cells (Wi-38)

Details on mammalian cell type (if applicable):

negative for viruses and mycoplasma

Additional strain / cell type characteristics:

not specified

Metabolic activation:

without

Test concentrations with justification for top dose:

0.1-10 µg/ml (Data taken from original report. In OECD SIDS the mentioned test concentration range is 1-1000 µg/ml.)

Controls

Untreated negative controls:

yes

Remarks:

0.85 % saline

Negative solvent / vehicle controls:

yes

Remarks:

0.85 % saline

True negative controls:

no

Positive controls:

yes

Positive control substance:

triethylenemelamine

Remarks:

Migrated to IUCLID6: 0.1 µg/l

Results and discussion

Test results

Species / strain:

mammalian cell line, other: human embryonic lung cells (Wi-38)

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not applicable

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

The low (0.1 µg/ml) and intermediate (1 µg/ml) levels contained 3 % and 4 % cells with acentric fragments, respectively. The intermediate level also contained a cell with a bridge. The negative control contained only one cell with an acentric fragment. While other levels were somewhat higher in aberrrations than the negative control, they are not considered significant. The positive control contained three cells with severely pulverizied chromosomes together with the other aberrations indicated in the summary sheet.