Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992-01-14 to 1992-06-02
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline-conform study under GLP without deviations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD 202 pt II, adopted April 1984.
Deviations:
no
Principles of method if other than guideline:
OECD Guideline for the Testing of Chemicals 202, Part II: "Reproduction Test at least 14 Days", Paris/France, adopted April 1984.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
not applicable

Test conditions

Hardness:
14.2 - 15.0 dH°
Test temperature:
19.3 - 20.4 °C
pH:
The initial pH of the systems was, on average, 7.9 for untreated, 7.8 and 7.9 for the treated samples. At the end of the exposure period, the pH for the respective concentrations were 8.4, 8.5 and 8.4.
Dissolved oxygen:
The initial oxygen concentration was, on average, 8.7 mg oxygen/l for untreated and for the treated samples. At the end of the exposure periods, the oxygen concentration of the respective samples were 8.3, 8.7 and 8.5 mg oxygen/l.
Salinity:
Conductivity 584 µs/cm
Nominal and measured concentrations:
nominal 0.625 mg/l: below lowest detected concentration of 1 mg/l
nominal 2.5 mg/l: mean measured 2.355 mg/l (94.2 % recovery rate)
nominal 5.0 mg/l: mean measured 4.559 mg/l (91.2 % recovery rate)
nominal 10.0 mg/l: mean measured 9.098 mg/l (90.1 % recovery rate)
recovery rates 79.6 % - 108.6 % of nominal concentration
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
immobilisation
Results with reference substance (positive control):
The quality of the animals used is checked by determining the EC 50 value for potassium dichromate twice per year, however no data reported.
Reported statistics and error estimates:
The results were statistically evaluated using the Steel-test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The NOEC- and LOEC-values for daphnia reproduction rate (21 days) were found to be ≥ 10 mg/l and >10 mg/l, respectively.