Reaction mass of Benzeneethanol, 2-chloro-α-(1-chlorocyclopropyl)-α-(chloromethyl)- and Oxirane, 2-(1-chlorcyclopropyl)-2-[(2-chlorphenyl)methyl]-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

HPLC

Details on sampling:

Sampling schedule of chemical analysis:
- Stock solution: on day 0, 1, 2, 3 ( Apr. 22, Apr. 23, Apr. 24 and Apr. 25, 2002)
- Control: on day 0, 1, 2, 3 (Apr. 22, Apr. 23, Apr. 24 and Apr. 25, 2002) in the freshly prepared medium; on day 1, 2, 3, 4 (Apr. 23, Apr. 24, Apr. 25 and Apr. 26, 2002) after 24 h of exposure
- Test concentrations: on day 0, 1, 2, 3 (Apr. 22, Apr. 23, Apr. 24 and Apr. 25, 2002) in the freshly prepared medium; on day 1, 2, 3, 4 (Apr. 23, Apr. 24, Apr. 25 and Apr. 26, 2002) after 24 h of exposure

Vehicle:

no

Details on test solutions:

A stock solution was prepared daily to give the desired series of test concentrations. To accelerate the solution procedure a five fold amount of the water solubility (20 mg/L) of the test substance under exposure conditions was added to 10 litres of dilution water on Apr. 21, 2002 and Apr. 22, 2002 and to 5 litres of dilution water on Apr. 23, 2002 and Apr. 24, 2002, then treated with an ultra turrax for 60 seconds at 8000 rpm and afterwards stirred for 24 h on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration using folded filters of pore size 7-12 μm.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Source: Di Mamma (Netherlands)
- Date of birth: Nov. 15, 2001
- Date supplied: March 28, 2002
- Acclimatisation: Stock held since March 28, 2002 and acclimatised to the test conditions since then
- Temperature: 20 - 24 °C
- Dissolved oxygen: > 5 mg/L
- Feeding: Commercial fish food, daily, Discontinued 24 h prior to test
- Mortalities during acclimatisation period: <2 %
- Medication: None
- Mean standard length (n = 60): 3.43 cm (S.D. = 0.19 cm)

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

12.6 °dH (224.9 mg/L CaCO3)

Test temperature:

21.5 - 22.3 °C

pH:

7.6 - 8.1

Dissolved oxygen:

7.7 - 8.9 mg/L

Salinity:

n.a.

Conductivity:

No data

Nominal and measured concentrations:

Test concentration/s (nominal): 1.25, 2.5, 5, 10 and 20 mg/L
Measured concentrations ranged from 80.6 - 107.6% of nominal values in the freshly prepared medium and from 27.1-70.3% of nominal values after 24 hours of exposure.

Details on test conditions:

Exposure conditions:
- Test vessels: glass aquaria holding 5 L of test media
- Experimental design: 5 test concentrations plus 1 control; 10 animals per test concentration; no feeding during the exposure period
- Method of initiation: animals were placed in prepared media
- Loading: 1.11 g body weight (wet weight) per litre
- Photoperiod: 16 h light: 8 h dark
- Temperature: mean +/- 1 °C (in the temperature range 20 - 24 °C)
- Aeration: gentle aeration via narrow glass tubes
- Method of administration: stock solution
- Medium renewal: daily
- Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 2, 24, 48, 72 and 96 hours, subjective assessments were made on the type and incidence of sub-lethal effects compared with control fish.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.88 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

3.46 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

6.87 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 80.6 - 107.6% of nominal values in the freshly prepared medium and from 27.1 - 70.3% of nominal values after 24 hours of exposure.

Validity criteria fulfilled:

yes

Remarks:

mortality in control <=10 %, dissolved oxygen concentration > 60 % of air saturation value throughout the test, pH should not vary by more than 1 unit

Conclusions:

After 96 hours of exposure of fish to the test substance an LC0 of 3.46 mg/L and an LC100 of 6.87 mg/Lwere determined. The geometric mean of the two values corresponding to the LC50 value is 4.88 mg/L. All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 80.6 - 107.6% of nominal values in the freshly prepared medium and from 27.1 - 70.3% of nominal values after 24 hours of exposure.

Executive summary:

The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. Groups of ten fish of the recommended size were exposed under semi-static conditions to a range of concentrations, nominally 1.25, 2.5, 5, 10 and 20 mg/L of test item dissolved in water for 96 h. Auxiliaries used to prepare the ·test media were an ultrasonic bath, a magnetic stirrer and folded filters.

During the test a temperature range of 20 - 24 °C is to be maintained in the test vessels, with a maximum temperature fluctuation of+/- 1 °C in each individual test. pH and oxygen values are measured at the beginning of the test and every 24 hours thereafter. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. After 96 hours of exposure an LC0 of 3.46 mg/L and an LC100 of 6.87 mg/Lwere determined. The geometric mean of the two values corresponding to the LC50 value is 4.88 mg/L.

All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 80.6 - 107.6% of nominal values in the freshly prepared medium and from 27.1 - 70.3% of nominal values after 24 hours of exposure.

Description of key information

The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. Groups of ten fish of the recommended size were exposed under semi-static conditions to a range of concentrations, nominally 1.25, 2.5, 5, 10 and 20 mg/L of test item dissolved in water for 96 h. Auxiliaries used to prepare the ·test media were an ultrasonic bath, a magnetic stirrer and folded filters.

During the test a temperature range of 20 - 24 °C is to be maintained in the test vessels, with a maximum temperature fluctuation of+/- 1 °C in each individual test. pH and oxygen values are measured at the beginning of the test and every 24 hours thereafter. Observations were made on the numbers of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 hours of exposure. After 96 hours of exposure both an LC 0 of 3.46 mg/L and an LC 100 of 6.87 mg/Lwere determined. The geometric mean of the two values corresponding to the LC 50 value is 4.88 mg/L.

All results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 80.6 - 107.6% of nominal values in the freshly prepared medium and from 27.1 - 70.3% of nominal values after 24 hours of exposure.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4.88 mg/L