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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1991-08-09
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP-Guideline study. According to the ECHA guidance document "Practical guide 6: How to report read-across and categories (March 2010)", the reliability was changed from RL1 to RL2.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Automatically generated during migration to IUCLID 6, no data available
IUPAC Name:
Automatically generated during migration to IUCLID 6, no data available
Details on test material:
- Name of test material (as cited in study report): Wessalith P
- Related CAS number: 1318-02-1
- Chemical name: Natriumaluminiumsilikat
- Framework: monoclinic
- Physical state: white crystalline powder
- Analytical purity: 80.6% active ingredient, 19.4% crystal water (source TNO), 79.8% active ingredient (source Degussa), respectively - two information sheets are given in the study report

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
As the test substance contained water insoluble particles, the centrifuged supernatants of each concentration were used for testing.

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h

Test conditions

Hardness:
no data
Test temperature:
21+/-0.5 °C
pH:
pH in the control at test start was 7.1, at test end 6.9 to 7.4 for neutralized background samples, and 7.1 to 10.2 for alkaline background samples, increasing with concentration.
Dissolved oxygen:
no data
Nominal and measured concentrations:
0, 0.1, 0.3, 0.10, 0.33, 1.0, 3.3, 5.5 and 10 g/L of the test substance
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
330 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
eluate
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
950 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
eluate
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
EC50
Effect conc.:
1 550 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
eluate
Basis for effect:
growth inhibition
Remarks on result:
other: alkalic

Any other information on results incl. tables

The EC10 was estimated to 0.33 g/L for both samples. The EC50 value for neutralized samples was found to correspond to 0.95 g/L (95% confidence interval of 0.57-1.59 g/L), for alkaline samples 1.55 g/L (95% confidence interval of 0.53-4.54 g/L).

The bacterial growth generally resulted in a decrease of the pH of the medium. The highest decrease was found with a neutralized test substance (0.33 g/L, pH 4.9). The inhibition was also stronger after neutralization, indicating that at least a part of the inhibition was caused by this decrease.

Applicant's summary and conclusion