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EC number: 200-184-4 | CAS number: 53-84-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jun.- Nov. 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Nadide
- EC Number:
- 200-184-4
- EC Name:
- Nadide
- Cas Number:
- 53-84-9
- Molecular formula:
- C21H27N7O14P2
- IUPAC Name:
- nadide
- Reference substance name:
- b-Nicotinamide-adenine Dinucleotide II (nadide)
- IUPAC Name:
- b-Nicotinamide-adenine Dinucleotide II (nadide)
- Details on test material:
- - Molecular formula (if other than submission substance): C21H27N7O14P2
- Molecular weight (if other than submission substance): 663.44
- Analytical purity: 98%
- Other: off-white powder
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: daphnia magna
- Source: in-house laboratory culture with a known history
- Age at study initiation (mean and range, SD): < 24 hours from parental daphnids of more than 2 weeks old.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Details on test conditions:
- Test vessel: 100 ml, all-glass
- No. of organisms per vessel: 5 per vessel containing 80 ml test solution
- Light intensity: 16 hours photoperiod daily
- Test concentrations: 0.1, 1.0, 10 and 100 mg/l- Reference substance (positive control):
- no
- Remarks:
- test medium without test substance or other additives
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- nadide did not induce acute immobilisation of Daphnia magna at the regulatory limit concentration, i.e. 100 mg/l after 48 hours of exposure (NOEC).
- Executive summary:
Acute Toxicity Study in Daphnia magna with nadide.
The study procedures described in this report were based on the OECD guideline No. 202, 2004. In addition, the procedures were designed to meet the test methods of the CommissionRegulation (EC) No440/2008,Part C.2, 2008 and the ISO International Standard 6341, 1996.
The batch of nadide tested was a off-white crystalline powder with a purity of 98% and completely soluble in test medium at the concentrations tested.
A combined limit/range-finding test was performed. Preparation started with a concentration of 100 mg/l. No other treatment than vigorous shaking was needed to completely dissolve the test substance. The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. The final test solutions were all clear and colourless.
Twenty daphnids per group (5 per vessel, 4 vessels) were exposed to a control and a concentration of 100 mg/l in a limit test. In addition, ten daphnids per group (5 per vessel, 2 vessels) were exposed to 0.1, 1.0 and 10 mg/l in a range-finding test. The total exposure period was 48 hours and samples for analytical confirmation of exposure concentrations were taken at the start and at the end of the test.
The measured concentration in the sample taken from the nominal concentration of 100 mg/l was in agreement with nominal and remained stable during the test.
Based on these results, the effect parameters were expressed in terms of analytically confirmed nominal concentrations.
The study met the acceptability criteria prescribed by the protocol and was considered valid.
nadide did not induce acute immobilisation of Daphnia magna at analytically confirmed nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).
The 48h-EC50was beyond the range tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/l.
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