Sodium 3-(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to guideline and under GLP

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Duration of test (contact time):

2 h

Initial conc.:

2.5 other: mg

Based on:

test mat.

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

TEST CONDITIONS
- Test temperature: 148 +/- 3°C

SAMPLING
- Sampling frequency: after 2h

CONTROL AND BLANK SYSTEM
- Blank: yes

Reference substance:

other: KHP

Parameter:

COD

Value:

1 479 other: mg/g O2

Results with reference substance:

COD = 204 mg/g O2 (criteria: 200 +/- 8 mg/g O2)

Validity criteria fulfilled:

yes

Conclusions:

The COD of the test substance is 1479 mg/g O2.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to guideline and under GLP, with some minor deviations

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Deviations:

yes

Remarks:

concentration of the test substance, method of O2-determination and inoculum (Husman)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

other: mixed culture of bacteria

Details on inoculum:

Test organism: mixed culture of bacteria (effluent of a Husman laboratory apparatus)

Duration of test (contact time):

5 d

Initial conc.:

1 mg/L

Based on:

test mat.

Initial conc.:

5 mg/L

Based on:

test mat.

Initial conc.:

10 mg/L

Based on:

test mat.

Initial conc.:

20 mg/L

Based on:

test mat.

Initial conc.:

50 mg/L

Based on:

test mat.

Initial conc.:

100 mg/L

Based on:

test mat.

Initial conc.:

200 mg/L

Based on:

test mat.

Initial conc.:

500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 23 +/- 2°C

SAMPLING
- Sampling frequency: after 5 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Reference substance:

benzoic acid, sodium salt

Remarks:

(purity: 99.3%)

Parameter:

BOD5

Value:

0 other: mg/g O2

Results with reference substance:

BOD5 = 100 (1040 ) mg/g for 4 (5) mg/L reference substance

BOD analytical data:

Substance	Concentration (mg/L)	O2-start	O2-end	Blank	BOD5 (mg/g O2)
Reference	4	8.8	3.9	0.5	1100
	5	8.8	3.1	0.5	1040
Test substance	1	8.9	8.4	0.5	/
	5	8.9	8.5	0.5	/
	10	8.9	8.4	0.5	/
	20	8.9	8.5	0.5	/
	50	8.9	8.4	0.5	/
	100	8.9	8.5	0.5	/
	200	8.9	8.4	0.5	/
	500	8.9	8.1	0.5	0.6

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The BOD5 of the test substance is 0 mg/g O2.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-03-15 to 1988-04-12

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No GLP stated

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (old version) (Ready Biodegradability: Modified AFNOR Test)

Deviations:

yes

Remarks:

Sterilization instead of steril filtration; Determination of bacteria concentration: by plating; Inoculum effluent of a domestic sewage treatment plant

Qualifier:

according to guideline

Guideline:

other: EEC Directive, Annex, C.4

Deviations:

yes

Remarks:

Sterilization instead of steril filtration; Determination of bacteria concentration: by plating; Inoculum effluent of a domestic sewage treatment plant

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): FAT-75`309/A

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic, non-adapted

Details on inoculum:

- Strain/Inoculation: Mixture of polyvalent bacteria colocted an 15.03.88 - effluent of a domestic sewage treatment plant (ARA-Rhein)
- Concentration of sludge: 1,7 X 10E7/ml

Duration of test (contact time):

28 d

Initial conc.:

45.3 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS

- Test temperature: 23 +/- 1°C
- Lighting: Indirect daylight
- estimation technique: TOC/DOC Shimadzu TOC-500
- Test concentration: 40mg/L DOC nominal

Reference substance:

other: glucose

Test performance:

Sampling: on days 0, 3, 7, 14, 28 days

Key result

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

NA

Results with reference substance:

Biodegradation corrected by the blank control and mesured as DOC (mg/L) is 87.5 % (28th day).

NA

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The biodegradation corrected by blank control and measured as DOC (mg/L) is 0 % (28th day). The test item is not readily biodegradable.

Executive summary:

Sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was tested in a biodegradation test according to OECD guideline 301A (modified Afnor-Test) using a concentration of nominally 40 mg/L DOC. The test was left running for 28 days. Glucose was chosen as positive control. Biodegradation of the positive control corrected by the blank control and measured as DOC (mg/L) was 87.5 % (28th day). The biodegradation of the test item corrected by blank control and measured as DOC (mg/L) is 0 % (28th day). No inhibiton on the activity of the bacteria was found. The test item is therefore evaluated as not ready biodegradable and not bacteric toxic at the test concentration of 40 mg/L.

Description of key information

Not readily biodegradable: 0% DOC removal in 28 days (OECD 301A)

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater

Additional information

Sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was tested in a biodegradation test according to OECD giudeline 301A (modified Afnor-Test) using a concentration of nominally 40 mg/L DOC. The test was left running for 28 days. Glucose was chosen as positive control. Biodegradation of the positve control corrected by the blank control and mesured as DOC (mg/L) was 87.5 % (28th day). The biodegradation of the test item corrected by blank control and measured as DOC (mg/L) is 0 % (28th day). No inhibiton on the activity of the bacteria was found. The test item is therefore evaluated as not ready biodegradable and not bacteric toxic at the test concentration of 40 mg/L.