Rape oil, sulfated, sodium salt

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Rape oil, sulfated, sodium salt

EC Number:

281-978-8

EC Name:

Rape oil, sulfated, sodium salt

Cas Number:

84082-30-4

Molecular formula:

not available (substance is a UVCB)

IUPAC Name:

Rape oil, sulfated, sodium salt

Details on test material:

- Name of test material : CP12
- Physical state: Liquid
- Lot/batch No.: 0012
- Expiration date of the lot/batch: 2015-08-01
- Storage condition of test material: Room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Nominal concentrations: 0, 1.0, 3.16, 10.0, 31.6 and 100.0 mg/mL
- Sampling method: Samples taken from each test vessel at test start and test end (after 72 hours)
- Sample storage conditions before analysis: No data

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Weighed aliquots of substance placed into brown glass flask and these filled with an appropriate amount of dilution water. Contents were stirred at 750 rpm for 24 hours at room temperature. After this a separation time of 1 hour was allowed before the water accomodated fraction was removed by siphoning from the approximate centre of the flask.
- Eluate: Test medium, as specified by OECD TG 201
- Controls: Yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): N/A
- Concentration of vehicle in test medium (final test solution(s) including control(s)): N/a
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None on visual inspection

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Desmodesmus subspicata CHODAT SAG 86.81
- Source (laboratory, culture collection): purchased at intervals from SAG Gottingen
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: A 4 day old pre-culture was used as inoculum, pipetted directly into each test vessel to give an initial cell density of 2 - 5E3 cells/mL).

ACCLIMATION
- Acclimation period: 4 days
- Culturing media and conditions (same as test or not): yes, OECD medium
- Any deformed or abnormal cells observed: No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

No

Test conditions

Hardness:

N/A, OECD medium used

Test temperature:

23 °C ± 2 °C

pH:

At test start = 7.9 8.0
At test end (72 hours) = 8.1 - 8.3

Dissolved oxygen:

No data

Salinity:

N/A OECD medium used

Nominal and measured concentrations:

Nominal concentrations: 0, 1.0, 3.16, 10.0, 31.6 and 100.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flask with a cotton wool plug which allowed ventilation.
- Material, size, headspace, fill volume: 100 mL OECD medium in a 250 mL Erlenmeyer flask
- Aeration: Rotary shaker at approximately 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A static
- Renewal rate of test solution (frequency/flow rate): N/A
- Initial cells density: 2-5 E3/mL
- Control end cells density: Control mean cell density increased 196-fold after 72 hours
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes - OECD medium
- Detailed composition if non-standard medium was used: N/A

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD medium
- Intervals of water quality measurement: 0 & 72h

OTHER TEST CONDITIONS
- Sterile test conditions: yes - filtered before use
- Adjustment of pH: no
- Photoperiod: continuous illumination:
- Light intensity and quality: 60 - 120 µE • m-2 • s-1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Growth inhibition 0, 24, 48 & 72h,
- Determination of cell concentrations: Fluorescence measurement 0, 24, 48 and 72 hours. Cell counting using a counting chamber at 72 hours
- Chlorophyll measurement: No

TEST CONCENTRATIONS
- Spacing factor for test concentrations: geometric series with a factor of 3.16
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study: Yes
- Test concentrations: 1.0, 10.0 and 100.0 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

17.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: CI 13.7-22.6 mg/L

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

70.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: CI 66.1-75.1 mg/L

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none
- Any stimulation of growth found in any treatment: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: Yes

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50: 0.590 mg/L
:

Reported statistics and error estimates:

The percentage inhibitions of the mean growth rate or yield based on the mean growth rate of the control were calculated for each concentration. The values which are close to x% were used for the determination of the ECx via linear interpolation using a plot with a logarithmic scale for the concentration versus the percentage of inhibition.
Dunnett’s test (one-sided) was carried out at a 95 % significance level, based on the assumption that each higher tested concentration must have at least the same or a stronger effect than the LOEC.

Any other information on results incl. tables

 

 

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions in which this study was conducted the EC50 values for inhibition of specific growth rate (ErC50) and yield (EyC50) were 70.4 mg/L and 17.4 mg/L respectively. The NOEC for inhibition of both specific growth rate (ErC0) and yield (EyC0) was 3.16 mg/L.

Executive summary:

Algal growth inhibition has been investigated in a 72 hour test according to OECD/EU test methods. The EC50 values for inhibition of specific growth rate (ErC50) and yield (EyC50) were 70.4 mg/L and 17.4 mg/L respectively. The NOEC for inhibition of both specific growth rate (ErC0) and yield (EyC0) was 3.16 mg/L.