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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 31, 1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: DIN 38 412
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: "BESTIMMUNG DER WIRKUNG VON WASSERINHALTSSTOFFEN AUF FISCHE - FISCHTEST (L15)"
Deviations:
no
GLP compliance:
no
Details on sampling:
- Concentrations: 100, 215, 464, 1000, 2150 and 4640 mg/l
- Sampling method: The product was added to the test water without any pretreatment
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden Orfe
- Source: Fischucht Eggers D-W2354 Hohenwestedt, FRG
- Length at study initiation (length definition, mean, range and SD): 7.3 cm (range: 6.8 - 8.1)
- Weight at study initiation (mean and range, SD): 3.7 g (range: 2.3 - 5.2)
- Method of breeding: ad libitum
- Food type: Growing feed F/B 50
- Corpulence factor: 0.9

ACCLIMATION
- Acclimation period: about 1 month
- Acclimation conditions (same as test or not): same as test
- Mortality during the last 2 weeks of housing: 0.1%
- Mortality during the adaptation period: 0%
- Medical treatment: a few days after arrival prophylactical treatment (twice with 0.05 mg/l malachite green chloride and once with 10 mg/l tetracycline hydrochloride)
- Adaptation to test water and test conditions: 3 days
- Withdrawal of food: 1 day before and during exposure
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
2.5 mmol/l
Test temperature:
20-21 °C
pH:
about 8.0
Dissolved oxygen:
> 60% of maximum saturation
Details on test conditions:
TEST SYSTEM
- Test vessel: All-glass aquarium (30 cm x 22 cm x 24 cm)
- Material, size, headspace, fill volume: flow-throught tank in tap water cleaned by active carbon
- Volume: 10 l
- Aeration: continuous aeration with oil-free air
- Photoperiod: 16h light and 8h darkness
- No. of organisms per vessel: 10


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria.
- Ca/mg ratio: 4:1
- Na/k ratio: 10:1
- Acid Capacity: 0.8 mmol/l



TEST CONCENTRATIONS
based on the results of a range finding study (LC 50 after 96 h: between 100 and 1000 mg/l) and a main study not evaluated the concentrations, spaced by a factor of about 2.2, were fixed as follows: 100, 215, 464 and 1000 mg/l.
Since no LC 100 had been achieved the additional concentrations 2150 and 4640 mg/l were added 1 week later.
Reference substance (positive control):
yes
Remarks:
chloroacetamide
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 792 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
ca. 215 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
ca. 215 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
ca. 2 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
Observed symptoms: Apathy, Narcotic-like state, Tumbling
Results with reference substance (positive control):
LC50 (48h) = ca. 32 mg/l
Reported statistics and error estimates:
Determination or calculation of the median lethal concentration (LC50) and, if possible the LC5 and the LC95 using the probit analysis[1] after hours (nominal conc): 1, 4, 24, 48, 72 and 96

[1]: "Probit analysis", Finney, d.j., Cambr. Univ. Press, 3rd ed., 1971
Sublethal observations / clinical signs:

Summary results:

LC50 (mg/l; nominal concentrations) after:
1h > 4600 mg/l
4h > 2200; < 4600 mg/l
24h > 1000; < 2200 mg/l
48h ca. 1000 mg/l
72h ca. 1000 mg/l
96h = 792 mg/l slope factor = 1.49
Validity criteria fulfilled:
not specified
Conclusions:
LC50 (96h) = 792 mg/l
Executive summary:

The substance to be registered has been tested for acute fish toxicity according to DIN 38 412 and has shown a NOEC = 215 mg/l, LC0 = 215 mg/l, LC50 = 792 mg/l and LC100 = 2150 mg/l.

Description of key information

Freshwater: LC50(96h) = 792 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
792 mg/L

Additional information

According to the CLP Regulation (EC) n. 1272/2008 ‘acute (short-term) hazard’ means for classification purposes the hazard of a substance or mixture caused by its acute toxicity to an organism during short-term aquatic exposure to that substance or mixture.

The test substance has been tested for acute fish toxicity. The selected key study shows a LC50 = 792 mg/l, this result is confirmed by the other available tests. The Huntsman test (1973) indicates a lower value (302 ppm after 96 h) but no complete information about the sample composition is available.

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