Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2014-06-13 - 2014-06-16
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Justification for data waiving:
other:

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test substance: 3-Amino-butan-1-ol
- Test substance No.: 13/0406-1
- CAS No.: 2867-59-6
- purity:93.6 corr. area-%. The test item is a racemate. The spectra show the expected signals for the given structure.

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
-Test species and strain: Desmodesmus subspicatus CHODAT (SAG 86.81)
- Age (on study day 0): A stock algal culture is maintained continuously at the test facility. Before the exposure an inoculum culture is prepared from the stock culture and incubated for 3 days at 21 – 24 °C (max. temperature difference 2 °C). After this time, the inoculum culture is in exponential growth phase and can be used to initiate the test (study day 0).
- Supplier: Collection of algal cultures in University of Göttingen/Germany

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Details on test conditions:
TEST SYSTEM
- Test concentrations: Control, 1, 10 and 100 mg/L (nominal concentration)
- Test volume: 10 mL
- Initial cells density: 0.5 x 104 cells/mL
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Addition of the stest substance: 112.4 mg of the test substance was stirred in 1L OECD medium for about 1 minute at room temperature. The stock solution was clear and the test substance was completely dissolved. The pH was 10.0 (pH of the OECD media was 8.0). The test concentrations were prepared by diluting the stock solution.

GROWTH MEDIUM
- Standard medium used: yes

Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 10 - < 100 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes