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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Readily biodegradable: 62% (CO2 evolution) in 28 days (OECD 301B)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

A valid study investigating the ready biodegradability of 2-Butenedioic acid (E)-, di-C18 -22-alkyl esters (CAS 68921-53-9) is available.The GLP study was conducted according to OECD 301B under aerobic conditions using non-adapted activated sludge as inoculum (Schwarz, 2013). An initial test concentration of 20 mg/L (TOC) was applied. Based on CO2 evolution a mean degradation of 62 % was observed after 28 days. According to “OECD Guidelines for the testing of chemicals – Revised introduction to the OECD guidelines for testing of chemicals, Section 3 – Part 1: Principles and strategies related to the testing of degradation of organic chemicals degradation” published by OECD (2006) the ready biodegradability data for mixtures should be evaluated carefully. For substances with different chain lengths the 10-day window should not be used for the interpretation of the results. It is possible that a sequential biodegradation occurred which might have led to a prolonged lag-Phase and therefore a slower biodegradation period.In this case only the pass level at 28 days has to be applied. Hence, the test substance is determined to be readily biodegradable according to OECD criteria.

In addition, the test was enhanced up to 60 days resulting in high overall biodegradation rates of 87 % biodegradation, showing that complete and ultimate will occur for this substance.