Registration Dossier
Registration Dossier
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EC number: 287-842-4 | CAS number: 85586-40-9
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
No effect on lemna growth was observed at a limint dose of 100 mg/L
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 100 mg/L
- EC10 or NOEC for freshwater plants:
- 100 mg/L
Additional information
A study was performed to assess the effect of the test item on the growth of the freshwater plant Lemna minor. The method followed that described in the OECD Guideline “Lemna sp. Growth Inhibition Test (March 2006)”.
Following a preliminary range-finding test, Lemna minor was exposed to an aqueous solution of the test item at concentrations of 1.0, 3.2, 10, 32 and 100 mg/L (three replicate flasks per concentration) for a period of 7 days, under constant illumination at a temperature of 24 ± 2°C.
The number of fronds in each control and treatment group was recorded on days 0, 2, 5 and 7 along with observations on plant development.
Chemical analysis of the test preparations on Day 0 (fresh media) and Day 7 (old media) showed measured test concentrations to be near nominal with the exception of the 1.0 mg/L test sample on Day 7 where a measured test concentration of 64% of nominal was obtained. Given that this test concentration was below the No Observed Effect Concentration (NOEC), this slightly lower than accepted value was considered to have had no impact on the outcome of the test.
Exposure of Lemna minor to the test item based on nominal test concentrations gave the following results:
Response Variable |
Measurement Variable |
EC50(mg/L) |
No Observed Effect Concentration (NOEC) (mg/L) |
Average Specific Growth Rate |
Frond Number |
>100 |
100 |
Dry Weight |
>100 |
100 |
|
Yield |
Frond Number |
>100 |
100 |
Dry Weight |
>100 |
100 |
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