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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic plants other than algae

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Administrative data

Link to relevant study record(s)

Description of key information

EC50 > 1000 mg/L

Key value for chemical safety assessment

EC50 for freshwater plants:
1 538 mg/L

Additional information

A test was performed on the similar substance 2 following OECD 221. The results show that the tested item is not toxic to Lemna minor with EC50 > 1000 mg/L.

Lemna minor is an aquatic plant that develops his leaves on the surface of the water, while nourishing substances are taken from the water solution. With this tests the observed effect is only related to the potential toxicity of the substance and not to the potential shading effect of a classical Alga study. A deviation to the protocol has been applied to the test recommended for dyes (Michael Cleuvers a, Hans-Toni Ratte, Phytotoxicity of coloured substances: is Lemna Duckweed an alternative to the algal growth inhibition test?Chemosphere 49 (2002) 9–15): "Beakers will be incubated on a black non-reflecting surface. Additionally, the walls of the incubation chambers will also be covered with black fabric in order to avoid reflection".

While for the same substance the “classical alga” test has provided a result of EC50 of about 14 mg/l (growing rate), with Lemna no toxicity up to the maximum tested level of 1000 mg/l on the active substance has been observed.

Therefore Direct Black 80 can be considered as not toxic to Lemna minor for Read Across with similar substance 2.