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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

EC50, 96h > 100 mg/l nominal (78 mg/l)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

One study was performed on the similar substance 3 (1997, Huntsman), following OECD 203, for acute toxicity on fish. Up to a nominal concentration of 100 mg/l (actual 78 mg/l) no mortalities of fish was observed while no effects could be observed due to the strong coloration of the test medium.

Therefore, according to Directive 93/21 EEC ( adaption to Directive 67/548/EEC ), the ecotoxicological classification of the tested substance is 'not toxic' to zebra-fish after an exposure period of 96 hours.

Direct Black 80 could be considered not toxic to fish for Read across with similar substance 3 and according to the QSAR carried out with the  prediction tool.