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EC number: 619-764-7 | CAS number: 173904-11-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-11-12 to 2011-10-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP study, in accordance to OECD, EU guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- updated 13th April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- dated May 30, 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling preparation:
Three replicate samples were taken from each test concentration and from the control at the start and at the end of the study.
Representative samples (in duplicate series) were taken at the start and at the end of the experiment. One series are stored frozen in the testing laboratory, one series were analysed immediately after sampling.
- Storage of the Samples: The second series of samples were stored in a refrigerator immediately after sampling, and were kept stored until delivery of the final report to enable additional analyses on request of the sponsor. After delivery of the final report, all samples will be discarded. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Formulation: At the preparation of the test item stock solution 50 mg test item was weighted and emulsified in 500 mL ISO Medium, thereafter sonicated for 30 minutes in ultrasonic bath (under cooling). The „stock solution” was centrifuged at 4000 rpm for 10 min. (20 °C) and filtrated using a 0.45 μm filter (VWR Vacuum Filtration System) and further diluted accordingly.
Untreated Control: ISO Medium with daphnids (without test item) was examined in parallel in the experiment. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection) 2100 Gödöllő, Kotlán S. u. 3. Hungary
- Age: Less than 24 hours old
- Sex: female
- Feeding, Aeration: None
- Acclimatization: The Daphnia mothers selected ca. one months prior to the start of the test were maintained in ISO medium under test conditions (temperature, light conditions, quality and content of reconstituted water, pH and total hardness).
The test animals were fed daily with a few mL of centrifuged algae (Pseudokirchneriella subcapitata) suspension.
To avoid the necessity of longer adaptation prior to the test, the water used in the test is similar (or the same) to the culture conditions (water temperature, background colour, light intensities). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 219.5 mg/L
- Test temperature:
- 21.1 – 21.9 °C in the climate chamber
21.4 – 21.6 °C in the test media - pH:
- pH 7.97 to 8.66 at the start of the test
pH 7.91 to 8.19 at the end of the test - Dissolved oxygen:
- 8. 37 to 8.83 mg/L at test start of the test
8.16 to 8.31 mg/L at test end of the test - Nominal and measured concentrations:
- Nominal 6.25; 12.5; 25, 50 and 100 mg/L
Mean measured concentrations with 95 % confidence interval: 5.46 ± 0.062, 13.3 ± 0.15, 27.1 ± 0.28, 57.5 ± 2.05, 113.1 ± 1.63 - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers of 50 mL volume with ~40 mL test medium
- No. of organisms per vessel: 20 Daphnia per control(s) and test concentration, divided into four groups of five animals, each group in at least 40 mL test medium
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark
EFFECT PARAMETERS MEASURED:
NOEC (48 h): No Observed Effect Concentration: highest test concentration at which no significant effect is determined after 48 hours test duration
EC 50: the calculated concentration of the test item which results in a 50 % immobilisation rate
EC 0: highest test concentration without a significant number of immobilised test animals
EC 100: the lowest test concentration at which all test animals are immobile
TEST CONCENTRATIONS
- Range finding study: Two non-GLP preliminary range-finding tests were performed. In the first range-finding test the examined nominal concentrations were: 3.125, 6.25, 12.5, 25, 50 and 100 mg test item/L. In the second range-finding test the same concentration range was investigated. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 68.79 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 56.88 - 85.19 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The actual 24-hours EC50 value of the potassium dichromate is: 2.05 mg/L.
This 24-hour EC50 value is in accordance with the accepted 0.6 – 2.1 mg/L range for Daphnia magna. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this acute daphnia toxicity study the observed endpoints for the effect of Incorez 397 were the followings: 48h EC50 value: 68.79 mg test item/L; 48h NOEC value: 25 mg test item/L and 48h LOEC value: 50 mg test item/L. All reported biological results are related to the nominal concentrations.
- Executive summary:
An acute toxicity test on Daphnia magna over an exposure period of 48 hours in a static system was assessed according to OECD Guideline 202 and EU method C.2. Five different concentrations of the test item Incorez 397 arranged in a geometric series and an untreated control group was tested in the main experiment. The concentrations were in nominal: 6.25; 12.5; 25, 50 and 100 mg/L. The 48-hour EC50value was determined as 68.79 mg/L (Probit analysis).The 48-hour NOEC was determined to be 25 mg/L, while the 48-hour LOEC was 50 mg test item/L. All reported biological results are related to the nominal concentrations.
Reference
The analytically determined mean test item concentrations in the analysed test media varied in the range from 86 % to 103 % of the nominal value at the start of the study and from 87 % to 116 % at the end of the study (see the attached analytical report). The measured concentrations remained within the range of ± 20% of nominal during the study, therefore, all reported biological results are related to the nominal concentrations applied in the test.
Description of key information
Under the conditions of this acute daphnia toxicity study the observed endpoints for the effect of Incorez 397 were the followings: 48h EC50 value: 68.79 mg test item/L; 48h NOEC value: 25 mg test item/L and 48h LOEC value: 50 mg test item/L. All reported biological results are related to the nominal concentrations.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 68.79 mg/L
Additional information
An acute toxicity Test on Daphnia magna over an exposure period of 48 hours in a static system was assessed according to OECD Guideline 202 and EU method C.2. Five different concentrations of the test item Incorez 397 arranged in a geometric series and an untreated control group was tested in the main experiment. The concentrations were in nominal: 6.25; 12.5; 25, 50 and 100 mg/L. The48-hour EC50value was determined as 68.79 mg/L (Probit analysis). The 48-hour NOEC was determined to be 25 mg/L, while the 48-hour LOEC was 50 mg test item/L. All reported biological results are related to the nominal concentrations.
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