Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012-08-16 - 2012-09-13
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report Date:
2012

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD Guideline 301 D (Ready Biodegradabality: Closed Bottle Test)
Deviations:
no
Principles of method if other than guideline:
In biodegradation test OECD 301 D the oxygen consumption corresponded to 30.6% of the ThOD after 14 days in the toxicity control. Therefore the test item can be considered not inhibitory to the inoculum according to this test guideline
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test organisms

Test organisms (species):
sewage, domestic
Details on inoculum:
Secondary effluent from a municipal wastewater treatment plant in Usserød, Denmark, wihich predominantly receives domestic sewage

Study design

Total exposure duration:
28 d

Test conditions

Reference substance (positive control):
yes
Remarks:
benzoic acid, sodium salt

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
other: oxygen consumption
Effect conc.:
30.6 other: % of ThOD
Nominal / measured:
meas. (not specified)
Conc. based on:
other: oxygen elektrode
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In biodegradation test OECD 301 D the oxygen consumption corresponded to 30.6% of the ThOD after 14 days in the toxicity control. Therefore the test item can be considered not inhibitory to the inoculum according to this test guideline
Remark: The test solution contains NaOH due to the production method.