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EC number: 939-409-2 | CAS number: 1329-99-3
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1.3 mg/L
Additional information
For the endpoint short-term toxicity to fish two studies are available: one Klimisch 1 key study and a supporting Klimisch 2 study. For the risk assessment the result from the more conservative Klimisch 1 study was used.
in the GLP Klimisch 1 study from Zok (1997) the toxicity of Depanol I to zebra fish was determined according to EU test guideline C.1 and OEC 203. The test was performed in two parts, first the concentrations from 10, 22, 50 and 100 mg/L and then from 1, 2.2, and 5 mg/L since there was 100 % mortality at 10 mg/L. At nominal 5 mg/L there was no mortality after 96 hours. Hence, the highest concentration without mortality was nominal 5 mg/L, corresponding to a measured concentration of 0.7 mg/L. Since the measured concentrations were significantly lower than the nominal, all values were based on mean measured concentrations. The 96 -hour LC50 was 1.3 mg/L and the 96 -hour LC100 was 2.4 mg/L. The discrepancy between nominal and mean measured concentrations is considered to be acceptable since the measured concentrations were used for the calculation of the endpoints. Therefore, the results of this study are considered relevant and acceptable for the risk assessment.
in the supporting GLP Klimisch 2 study from Markert and Jung (1988) the toxicity of Depanol J to zebra fish was determined according to EU test guideline C.1 and OEC 203. The test was performed in four parts, first the concentrations of 125 and 250 mg/L then 10 mg/, then 35 and 50 mg/L and at last 18 and 25 mg/L. Dose-verification analysis was not performed. In all treatment groups relevant for the determination of the LC50, the test item was reported to be in solution. At 18 mg/L a slight turbidity was observed after 48 hours. At 125 and 250 mg/L test item was floating on the surface of the test solutions. At nominal 10 mg/L there was no mortality after 96 hours. Hence, the highest concentration without mortality was nominal 10 mg/L. The 96 -hour LC50 was 27.6 mg/L and the 96 -hour LC100 was 50 mg/L. The endpoints were based on nominal concentrations. Due to the lack of dose-verification analysis, the exposure concentrations are not clear. Therefore, the results of this study are considered to have minor relevance for the risk assessment.
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