Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

Currently viewing:

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 1 OCT 1979 to 15 OCT 1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given: comparable to guidelines

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
: no individual data included in report
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
EC Number:
254-100-6
EC Name:
Dichloro-5,12-dihydroquino[2,3-b]acridine-7,14-dione
Cas Number:
38720-66-0
Molecular formula:
C20H10Cl2N2O2
IUPAC Name:
1,8-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione; 1,9-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione; 2,9-dichloro-5,7,12,14-tetrahydro-5,12-diazapentacene-7,14-dione

Test animals

Species:
rat
Strain:
other: Hoe WISKf (SPF71)
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: in-house breeding
- Weight at study initiation: 180 to 202 g
- Fasting period before study: 16 hours
- Housing: in plastic cages in groups
- Diet: Altromin 1324 (Altromin GmbH, Lage/Lippe, Germany), ad libitum
- Water. tap water, ad libitum

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: sesame oil
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 25 % suspension (w/v)
Doses:
10000 mg/kg bw
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
> 10 000 mg/kg bw
Remarks on result:
other: no animals died within the 14 days observation period
Mortality:
- no deaths occurred
Clinical signs:
other: - post application faeces was stained red
Gross pathology:
- in animals sacrificed at the end of the observation period no macroscopically visible changes were found

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: other: CLP regulation
Conclusions:
Single application of the limit dose of 10000 mg test item per kg bw did not cause lethality in female rats during the 14 days observation period, resulting in a LD50 > 10000 mg/kg bw.
Executive summary:

Female rats were subjected to test acute oral toxicity. The test item was administered at the limit dose of 10000 mg/kg bw to 10 rats. During the 14 days observation period no animals died and there were no changes found in necropsy, thus leading to a median lethal dose (LD50) > 10000 mg/kg bw.

Therefore, the test item has not to be classified for acute oral toxicity according to Regulation (EC) No 1272/2008.