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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro DNA damage and/or repair study
Remarks:
Type of genotoxicity: gene mutation
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
Not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Document insufficient for assessment.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970

Materials and methods

Principles of method if other than guideline:
Method: Induction of mitotic gene conversion or non-reciprocal recombination was studied with the diploid strain D4 of Saccharomyces cerevisiae.
GLP compliance:
not specified
Type of assay:
mitotic recombination assay with Saccharomyces cerevisiae

Test material

Constituent 1
Reference substance name:
Zinc sulphate
EC Number:
231-793-3
EC Name:
Zinc sulphate
Cas Number:
7733-02-0
IUPAC Name:
zinc sulfate
Details on test material:
- Name of test material (as cited in study report): Zinc sulfate

Method

Target gene:
Gene conversion was studied at the following two loci: ade2 and trp5.
Species / strain
Species / strain / cell type:
Saccharomyces cerevisiae
Details on mammalian cell type (if applicable):
Not applicable
Additional strain / cell type characteristics:
not applicable
Metabolic activation:
not applicable
Metabolic activation system:
Not applicable
Test concentrations with justification for top dose:
Optimal or highest concentration (ppm): 5,000/1,000
Vehicle / solvent:
0.1M potassium phosphate buffer (pH 7.5)
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
yes
Remarks:
0.1 M potassium phosphate buffer (pH 7.5)
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
METHOD OF APPLICATION: in suspension

DURATION
- Exposure duration: 4 h
Evaluation criteria:
No data
Statistics:
No data

Results and discussion

Test results
Species / strain:
Saccharomyces cerevisiae
Metabolic activation:
not applicable
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not determined
Vehicle controls validity:
not applicable
Untreated negative controls validity:
not applicable
Positive controls validity:
not applicable
Additional information on results:
None
Remarks on result:
other: strain/cell type: diploid strain D4
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Table 1: Zinc sulfate tested for the induction of mitotic gene conversion in strain D4 of Saccharomyces cerevisiae

Convertants per 106survivors at the loci

Survivors (%)

Optimal or highest concentration (ppm)

ade2

trp5

45.7 (28.5)

2.9 (3.3)

97

5000/1000

Doses are presented at which the highest frequencies of mitotic gene convertants per survivor were observed. Those concentrations were sometimes different for conversion at the two loci. The corresponding control values are given in parentheses. Treatments were performed in 0.1M potassium phosphate butter (pH 7.5) with shaking for 4 h at 25 ± 0.1 °C.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test material was considered to be non-convertogenic under the conditions of this test.
Executive summary:

A study was conducted to determine the potential of the test material to induce mitotic gene conversion using diploid strain D4 of Saccharomyces cerevisiae. 

Diploid strain D4 (MD 20:a,+,ade2-I, trp5-27-+) was treated with the test material for 4 h in selective synthetic media (WICKERHAM), supplemented with amino acids and nucleobases (ROMAN) and solidified with 1.5% Difco bactoagar, usually up to 5,000 ppm/plate.

The test material showed inability to induce mitotic gene conversion. Convertants per 106survivors at the loci ade2 & trp5 was 45.7 & 2.9, respectively.

 

The test material was considered to be non-convertogenic under the conditions of this test.

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