Zinc sulfide (ZnS), copper chloride-doped

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

Not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Document insufficient for assessment.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Materials and methods

Principles of method if other than guideline:

Method: Induction of mitotic gene conversion or non-reciprocal recombination was studied with the diploid strain D4 of Saccharomyces cerevisiae.

GLP compliance:

not specified

Type of assay:

mitotic recombination assay with Saccharomyces cerevisiae

Test material

Test material information

Constituent 1

Reference substance name:

Zinc sulphate

EC Number:

231-793-3

EC Name:

Zinc sulphate

Cas Number:

7733-02-0

IUPAC Name:

zinc sulfate

Details on test material:

- Name of test material (as cited in study report): Zinc sulfate

Method

Target gene:

Gene conversion was studied at the following two loci: ade2 and trp5.

Species / strain

Species / strain / cell type:

Saccharomyces cerevisiae

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not applicable

Metabolic activation:

not applicable

Metabolic activation system:

Not applicable

Test concentrations with justification for top dose:

Optimal or highest concentration (ppm): 5,000/1,000

Vehicle / solvent:

0.1M potassium phosphate buffer (pH 7.5)

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

0.1 M potassium phosphate buffer (pH 7.5)

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF APPLICATION: in suspension

DURATION
- Exposure duration: 4 h

Evaluation criteria:

No data

Statistics:

No data

Results and discussion

Test results

Species / strain:

Saccharomyces cerevisiae

Metabolic activation:

not applicable

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

not applicable

Untreated negative controls validity:

not applicable

Positive controls validity:

not applicable

Additional information on results:

None

Remarks on result:

other: strain/cell type: diploid strain D4

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Table 1: Zinc sulfate tested for the induction of mitotic gene conversion in strain D4 of Saccharomyces cerevisiae

Convertants per 10^6survivors at the loci		Survivors (%)	Optimal or highest concentration (ppm)
ade2	trp5
45.7 (28.5)	2.9 (3.3)	97	5000/1000

Doses are presented at which the highest frequencies of mitotic gene convertants per survivor were observed. Those concentrations were sometimes different for conversion at the two loci. The corresponding control values are given in parentheses. Treatments were performed in 0.1M potassium phosphate butter (pH 7.5) with shaking for 4 h at 25 ± 0.1 °C.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The test material was considered to be non-convertogenic under the conditions of this test.

Executive summary:

A study was conducted to determine the potential of the test material to induce mitotic gene conversion using diploid strain D4 of Saccharomyces cerevisiae. 

Diploid strain D4 (MD 20:a,+,ade2-I, trp5-27-+) was treated with the test material for 4 h in selective synthetic media (WICKERHAM), supplemented with amino acids and nucleobases (ROMAN) and solidified with 1.5% Difco bactoagar, usually up to 5,000 ppm/plate.

The test material showed inability to induce mitotic gene conversion. Convertants per 10⁶survivors at the loci ade2 & trp5 was 45.7 & 2.9, respectively.

 

The test material was considered to be non-convertogenic under the conditions of this test.