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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

The activated sludge respiration inhibition of the test substance, TM 09-217, was assessed according to OECD Test Guideline 209 using a respiration test (carbon and ammonium oxidation) method. The nominal TM 09-217 concentrations used in the study were 10, 100 and 1000 mg/L; a single replicate was tested at 10 mg/L and 100 mg/L and three replicates at 1000 mg/L. The control consisted of two replicates. Concentrations of TM 09-217 were measured using gas chromatography with mass spectrometric detection (GC-MS). In samples of freshly prepared medium prior to incubation, measured concentrations of TM 09-217 had been adequately achieved ranging between 87 and 112% of their nominal values. At the end of incubation (thirty minutes) measured concentrations of TM 09-217 decreased, ranging between less than the limit of detection to 43% of their nominal values (between 0 and 46% of their starting values). Based on a geometric mean, the mean measured concentrations of TM 09-217 were 1.47, 57.1 and 650 mg/L, and these values have been used in the assessment of inhibition of the test substance. At the end of the incubation period, levels of inhibition were 0%, 51% and 79% (mean) at 1.47, 57.1 and 650 mg/L respectively, The EC50 of TM 09-217 was found to lie within the range 10 to 100 mg/L (geometric mean measured concentration) and NOEC was 1.47 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
10 mg/L

Additional information