Registration Dossier
Registration Dossier
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EC number: 202-551-4 | CAS number: 97-00-7
Specific investigations: other studies
Administrative data
- Endpoint:
- cytotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Publication, main features reported, non-standard method
Data source
Reference
- Reference Type:
- publication
- Title:
- Differential effects of raising and lowering intracellular GSH levels on the cytotoxicity of allyl isothiocyanate, tert.-butylhydroperoxide and chlorodinitrobenzene
- Author:
- Bruggemann, I. M.; et al
- Year:
- 1 988
- Bibliographic source:
- Toxic. in vitro 2: 31-35 (1988)
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- cloning efficiency of Chang cells was assessed in presence of increased or reduced intracellular glutathione levels
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- 1-chloro-2,4-dinitrobenzene
- EC Number:
- 202-551-4
- EC Name:
- 1-chloro-2,4-dinitrobenzene
- Cas Number:
- 97-00-7
- Molecular formula:
- C6H3ClN2O4
- IUPAC Name:
- 1-chloro-2,4-dinitrobenzene
- Details on test material:
- commercial sample of DNCB
Constituent 1
Test animals
- Species:
- human
- Strain:
- other: epithelial like human liver cells (chang cells)
- Sex:
- not specified
Administration / exposure
- Route of administration:
- other: in vitro
- Vehicle:
- not specified
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- no data
- Frequency of treatment:
- single, 2 h
- Post exposure period:
- 10 days
- No. of animals per sex per dose:
- no applicable
- Details on study design:
- cells were pretreated with glutathione ethyl ester to increase intracellular glutathione (GSH) or with diethyl maleate to reduce intracellular GSH. Afterwards cells were treated with DNCB and the cloning effinciency was determined.
Examinations
- Examinations:
- Cloning efficiency after 2 h treatment and subsequent 10 days incubation at 37 C.
Results and discussion
- Details on results:
- Increased GSH-levels gave rise to increased cytotxicity (reduced cloning effficiency, while reduced GSH reduced the toxci effects of DNCB in these cells.
Applicant's summary and conclusion
- Conclusions:
- The toxic mechanism of DNCB in a human liver cell line seems to envolve GSH in activation of DNCB. Cytotoxicity was found inversely proportional to GSH concentration in vitro.
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