Reaction mass of ethoxylated N-oleyl-1,3-propanediamine, hydrofluorides of and ethoxylated N-palmityl-1,3-propanediamine, hydrofluorides of and ethoxylated N-stearyl-1,3-propanediamine, hydrofluorides of and olaflur

Short-term toxicity to aquatic invertebrates

Description of key information

In the 48-hour static acute toxicity test to Daphnia magna with Olaflur the 48-hour EC50 value was determined as 327 ug test item/L based on nominal concentration (Probit analysis). The overall NOEC was determined to be 125 ug/L nominal.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.327 mg/L

Additional information

The purpose of this study was to evaluate the influence of the test item Olaflur on the mobility respectively survival of Daphnia magna. Young Daphnia were exposed in a static test to the test item for 48 hours, added to test water at a range of concentrations. The test item concentration of the test solutions was measured by HPLC/MS/MS method at the start and at the end of the test. The nominal test item concentrations were 0.063; 0.125; 0.25; 0.5, 0.75 and 1 mg test item/L (63; 125; 250; 500; 750 and 1000 ug/L). The highest nominal concentration was 1 mg/L (1000 ug/L). At the start of the test the measured concentrations at the nominal concentrations of 0.125, 0.25, 0.5, 0.75 and 1 mg/L were above the quantification limit (the limit of quantification of the analytical method was 19.3 ug/L that equivalent with 0.0193 mg/L) of the analytical method; however, these measured test item concentrations were 31-64 % of the nominal only. Due to the adsorptive properties of Olaflur, pre-conditioning of the test vessels was necessary. In the main experiment, results obtained from reference samples (without Daphnia), in which a good recovery was noted even at the end of the test period, supported the assumption that the test item adsorbs to the biological material (the daphnids). So, these additional investigations revealed that the low recovery was due to the presence of test organisms and not due to limited homogeneity or stability in the test medium. This is also supported by the observations made in the long-term Daphnia study with the free amine base of the fluoride salt (= Olaflur). This leads to the conclusion that the organisms were fully exposed to the bulk concentration of the test substance during the test. Thus, all effect values are therefore given based on the nominal test item concentrations (for details see Section 6.1.4).