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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
other information
Study period:
23 July 2009
Reliability:
1 (reliable without restriction)

Data source

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: The Ocular Toxicity Working Group (OTWG) of the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) and the National Interagency Centre for the Evaluation of Alternative Toxicological Methods (NICEATM), Background Review D
Qualifier:
according to guideline
Guideline:
other: In Vitro Techniques in Toxicology Database (INVITTOX) protocol 124. Bovine Opacity and Permeability Assay - SOP of Microbiological Associates Ltd., 1999.
Qualifier:
according to guideline
Guideline:
other: Gautheron P., Dukic M., Alix D. and Sina J.F., Bovine corneal opacity and permeability test: An in vitro assay of acular irritancy. Fundam Appl Toxicol 18:442-449, 1992.
Qualifier:
according to guideline
Guideline:
other: Organisation for Economic Co-operation and Development (OECD), OECD Guidelines for Testing of Chemicals; Draft proposal for a new guideline: "Bovine Corneal Opacity and Permeability (BCOP) Test Method for Identifying Ocular Corrosives and Severe Irritants
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1-[3-(2-methoxy-5-nitrophenoxy)propyl]-4-methylpiperazine
EC Number:
700-445-7
Cas Number:
846023-54-9
Molecular formula:
C15H23N3O4
IUPAC Name:
1-[3-(2-methoxy-5-nitrophenoxy)propyl]-4-methylpiperazine

Test animals / tissue source

Species:
other: Not applicable
Strain:
other: Not applicable

Test system

Vehicle:
water
Controls:
other: Not applicable
Duration of treatment / exposure:
Not applicable
Observation period (in vivo):
Not applicable
Number of animals or in vitro replicates:
Not applicable

Results and discussion

In vivo

Results
Irritation parameter:
other: Bovine Corneal Opacity and Permeability
Score:
ca. 6.3
Remarks on result:
other: after 240min of treatement

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information
Conclusions:
WAY-190192-A induced mild ocular irritation through both endpoints, resulting in a mean in vitro irritancy score of 6.3 after 240 minutes of
treatment.
Finally, it is concluded that this test is valid and that WAY-190192-A is a mild irritant in the Bovine Corneal Opacity and Permeability test under the experimental conditions described in this report.