Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20.06.2006 - 23.06.2006
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): fluorphenylepoxyethan (FOX)
- Physical state: liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 1, 2, 5, 10, 20, 50, 100 ml WAF/l
- Sampling method: 2 x 50 ml of stock solution
- Sample storage conditions before analysis: Freezer a -20ºC

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Skeletonema costatum
Details on test organisms:
TEST ORGANISM
- Common name: Diatom algae. (NIVA BAC 1)
- Source (laboratory, culture collection): DHI, Water & Enviroment
- Method of cultivation: stock culture

ACCLIMATION
- Acclimation period: None
- Culturing media and conditions (same as test or not): same

Study design

Water media type:
saltwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
19ºC
pH:
8
Salinity:
28 ‰
Nominal and measured concentrations:
Stock solution made by 500 mg substance dissolved in 500 ml test media.
Nominal concentration: 1000 mg/l.
Substance was not fully dissolved, therfore a test was taken from the middle of the volumetric flask (water accomodated fraction = WAF)
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol (3,5-DCP), CAS No. : 591-35-5

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
0.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.22 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.48 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
biomass
Details on results:
pH in control was > 1 (1.2) but this is assessed not to have influence on the result.
Results with reference substance (positive control):
- Results with reference substance valid: Yes
- EC50: 1.63 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Cell density increased by a factor of more than 16 during test, results for reference substance within acceptable range, 1.49-1.78 mg/l %
Conclusions:
EC50(72 h) of fluorphenylepoxyethan (FOX), CAS No.: 88374-05-4 is 0.48 mg/L for Skelotonema costatum.