N-[3-(dimethoxymethylsilyl)-2-methylpropyl]ethylenediamine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

yes

Analytical monitoring:

no

Details on test solutions:

The test substance was dissolved in double distilled water. Stock solution: 0.625 g/l

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test temperature:

21.7 - 21.9 °C

Nominal and measured concentrations:

Test substance concentrations of 50, 250, 125, 62.5, 31.3, 15.6 mg/l 

Details on test conditions:

Sterile 250-ml Erlenmeyer flasks with ground glass stoppers.
6 growth controls (containing the bacterial suspension)
6 control (test substance and no bacterial suspension)
3 parallel flasks for each of the 6 test concentrations (and bacterial suspension)
1 pH control for each of the 6 test concentrations (and bacterial suspension)

Reference substance (positive control):

no

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

25 mg/L

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

67 mg/L

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Table 1: Controls and test substance: pH, absorption at 432 nm, and percentage inhibition of the growth rate.

Flask	Loading rate (mg/l)	Absorption at 436 nm	% Inhibition growth rate	pH
Test substance (average of three)	500	0.034	99.0	9.4
Test substance (average of three)	250	0.062	97.4	8.4
Test substance (average of three)	125	0.243	87.0	7.6
Test substance (average of three)	62.5	0.998	43.7	7.3
Test substance (average of three)	31.3	1.529	13.3	7.2
Test substance (average of three)	15.6	1.737	1.3	7.1
Control (average of six)	-	1.760	-

 

Conclusions:

A 16hr EC50 value of 67 mg/l and EC10 value of 25 mg/l were determined for toxicity to pseudomonas putida (growth rate) in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP.

Reference 2

Endpoint:

toxicity to microorganisms

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to the attached justification for grouping of substances provided in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

25 mg/L

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

67 mg/L

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Description of key information

Toxicity to microorganisms: EC50 of 67 mg/l; EC10 25 mg/l (Pseudomonas putida, growth rate).

Additional information

There are no microorganism toxicity data available for N-[3-(dimethoxymethylsilyl) -2-methylpropyl]ethylenediamine (CAS 23410-40-4), therefore good quality data for the analogue substance, N-(3-(trimethoxysilyl) propyl) ethylenediamine (CAS 1760-24-3) have been read across. Both substances share the comparable silanol hydrolysis products, N-[3-(dihydroxymethylsilyl)-2-methylpropyl]ethylenediamine and N-[3 (hydroxydimethylsilyl)propyl]ethylenediamine. It is therefore considered valid to read-across the results for N-(3-(trimethoxysilyl) propyl) ethylenediamine to fill the data gap for the registered substance. The other hydrolysis product being methanol, which does not have adverse effects on microorganisms (OECD, 2004a).

Both substances are within a wider analogue group than the analogue group discussed in Section 1.4. Substances within this wider analogue group, in general, exhibit no evidence of significant toxicity to microorganisms.

This wider analogue group for the toxicity to microorganisms endpoint consists of a number of sub-classes of substances. However, read-across is carried out between substances with the same sub-class in most cases.

Table 7.1.6 presents microorganism toxicity data available for substances within the sub-class (III-8) of substances having amino group(s) in side chain after any very rapid hydrolysis has been accounted for.Both N-[3-(dimethoxymethylsilyl) -2-methylpropyl]ethylenediamine (CAS 23410-40-4) and N-(3-(trimethoxysilyl) propyl) ethylenediamine (CAS 1760-24-3) are members of this sub-class.

Table 7.1.6. Microorganism toxicity data available for substances within the relevant sub-class (III-8) of chemicals

CAS	Name	Result: E(I)C50(mg/l)	Result: NOEC (or EC10/ EC20) (mg/l)	Guideline Number	Test method	Species	Duration	Reliability
027445-54-1	Cyclohexanamine,N-[(diethoxymethylsilyl)methyl]-	624.2 mg/l    (EC20 240.2 mg/l)		OECD 209	ASRI		3hr - but stirred 24hr	1a
003068-78-8	N-[3-(trimethoxysilyl)propylcyclohexylamine]	>1000	EC20 = 203 mg/l	OECD 209	ASRI		3 hr	1a
157923-74-5	2,2-dimethyl-4-(trimethoxysilyl)butylamine	>1000	EC15 869. 17.5% inhibition at 1000 mg/l.	OECD 209	ASRI		3 hr	1a
227085-51-0	N-ethyl-3-trimethoxysilyl-2-methylpropanamine	>1000		OECD 209	ASRI		3 hr	1a
000919-30-2	3-aminopropyltriethoxysilane	43 mg/l	EC10 13 mg/l	Huls AG method	oxygen consumption	P. putida	5.75 hr	2c
082985-35-1	Bis(trimethoxysilylpropyl)amine	estimated value, approximately 1000	220	OECD 209	ASRI		3 hr	1a
001760-24-3	N-(3-(trimethoxysilyl)propyl)ethylenediamine	67 mg/l	EC10 25 mg/l	DIN 38 412, Part 8 (Pseudomonascell multiplication inhibition test)	growth inhibition test	P. putida	16 hr	1c
	[N,N-Bis(trimethylsilyl)aminopropyl]methyldiethoxysilane	>100		OECD 209	ASRI		3 hr	1a
None	reaction mix (reaction mass of 6 benzylated amine silanes)	569	130; EC20 172	OECD 209; EC C.11	ASRI		3 hr	1a

 

Additional information is given in a supporting report (PFA, 2013l) attached in Section 13 of the IUCLID dossier.

The influence of the test item N-(3-(trimethoxysilyl) propyl) ethylenediamine (CAS 1760-24-3) were determined for toxicity to Pseudomonas putida (growth rate) in a reliable study conducted according to DIN 38412/8 and GLP (Hüls AG, 1994c). The nominal test concentrations were 15.6, 31.3, 50, 62.5, 125, 250 mg/l. A 16 hr EC₅₀ value of 67 mg/l and EC₁₀ value of 25 mg/l was determined in a static test type.